摘要
Effects of providing substrates containing corn silage (12 g of DM/day) and lucerne hay (3 g DM/day) to an artificial rumen (RUSITEC) in which the whole plant corn was not inoculated (CS, control), or inoculated with Lactobacillus plantarum CCM 4000 (CS + LP), Lactobacillus fermentum LF2 (CS + LF) or Enterococcus faecium CCM 4231 (CS + EF) on rumen metabolism was examined. The inoculants were promising probiotic- and bacteriocin-producing strains. The substrates were fermented in fermentation vessels for 11 days, which included a stabilization period of 5 days. The counts of inoculants decreased during ensiling of corn, and at the end of ensiling (105 days), their counts were less than 1.0 log 10 cfu/g; probably for lower pH (3.44–3.54). Organic matter and neutral detergent fibre degradabilities were increased in inoculated CS compared to control. Ammonia N production was decreased in all inoculated substrates. From other fermentation parameters, only total VFA production, production of acetate, n-butyrate (mmol/day) significantly increased during fermentation of CS + LP and CS + LF substrates compared to control. The inoculated CS substrates influenced the efficiency of microbial protein synthesis (P<0.001). Biohydrogenation of C 18:1, C18:2 and total C18 FA increased in all inoculated CS substrates in fermentation fluid in RUSITEC. The concentration of trans 11 C18:1 (TVA) was unchanged in inoculated CS substrates and the concentration of cis 9, trans 11 C18:2 (CLA) were decreased (P<0.05) compared in CS. All inoculants of whole plant corn were not effective for increasing CLA and TVA concentration in fermentation fluid and for decreasing of rumen biohydrogenation of C18 fatty acids in RUSITEC.
摘要译文
效果人工瘤胃（RUSITEC）其中整个植物玉米未接种提供含有玉米青贮（12克的DM /天）和苜蓿干草（3克的DM /天）底物（CS，对照），或接种对瘤胃代谢的植物乳杆菌CCM 4000（CS LP），发酵乳杆菌LF2（CS LF）或肠球菌CCM 4231（CS EF）进行了检查。该接种是有希望probiotic-和细菌生产菌株。将基板发酵中发酵容器为11天，其中包括5天的稳定化周期。接种剂的计数青贮玉米中下降，并在青贮（105天）的端部，其计数少于1.0日志10个/克;可能是较低的pH值（3.44-3.54）。有机物和中性洗涤纤维降解率与对照组相比在接种CS均升高。氨氮生产的所有接种的基板下降。从其他发酵参数，只有总VFA生产，生产醋酸，正丁酸盐（毫摩尔/天）的CS LP和对照相比的CS LF基板的发酵期间显著增加。将接种的CS基板影响的微生物蛋白质合成的效率（PC18：2和C18总量增加FA在所有接种CS基板在发酵液中RUSITEC。反11 18的浓度：1（TVA）持平在接种的CS基板和顺9的浓度，反式11 C18：2（CLA）被降低（P全厂所有的玉米接种没有有效地提高CLA和TVA浓度发酵液，在RUSITEC减小C18脂肪酸瘤胃生物氢化的。
Dušan Jalč[a]; Zora Váradyová[a]; Andrea Lauková[a]; Petr Homolka[b]; Filip Jančík[b]. Effect of inoculated corn silage on rumen fermentation and lipid metabolism in an artificial rumen (RUSITEC)[J]. Animal Feed Science and Technology, 2009,152(3-4): 256–266