摘要
We previously reported that AI with frozen-thawed boar semen supplemented with caffeine increased the number of uterine sperm by inhibiting migration of polymorphonuclear leukocytes (PMNs) into the uterine lumen, and also improved fertility of gilts and sows. The objective of the present study was to determine the effects of the addition of caffeine to a thawing solution on postthaw sperm quality and uterine inflammatory response after AI with frozen-thawed boar semen. Incubation of frozen-thawed sperm in Modena solution supplemented with 10 mM caffeine for 90 minutes improved (P < 0.05) percentages of progressive motility, straightness, and linearity of sperm movement compared with no caffeine, without causing damage to plasma or acrosomal membranes. Gilts inseminated once with 2 × 109 frozen-thawed sperm suspended in Modena solution with or without caffeine, and gilts that did not receive AI, were slaughtered 4 hours later. Uteri were recovered for analysis of number of uterine PMNs and mRNA expression (quantitative reverse transcription polymerase chain reaction) of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-8, monocyte chemoattractant protein-1, and cyclooxygenase 2 in the endometrium. Caffeine decreased (P < 0.05) both the number of total uterine PMNs and expression of IL-8 mRNA in the endometrium after AI. The amount of IL-8 and cyclooxygenase 2 mRNA after AI in the absence of caffeine were higher than samples from gilts that did not receive AI (P < 0.05), whereas there were no significant differences between treatments in expression levels of tumor necrosis factor-α, IL-1β, IL-6, or monocyte chemoattractant protein-1 mRNA. Pregnancy rate in sows inseminated with sperm supplemented with caffeine (16 of 23; 70%) tended (P < 0.1) to exceed that without caffeine (12 of 26; 46%), but litter size was not affected. In conclusion, the addition of caffeine to the thawing solution inhibited migration of uterine PMNs, probably by downregulating IL-8 mRNA expression in the endometrium.
摘要译文
LY报道，人工智能与补充有咖啡因冻融公猪精液通过抑制多形核白细胞（PMN）的迁移入子宫腔增大的子宫精子数目，同时也提高了母猪和经产母猪的生育能力。Ë本研究是为了确定在加入咖啡因对postthaw精子质量和子宫炎症反应解冻溶液的AI后用冻融公猪精液的影响。冻融精子摩德纳溶液补充了10mM咖啡因90分钟提高孵化（P和无咖啡因精子运动的线性比较，而不会损坏血浆或顶体膜。母猪2×10次授精SUP 9 / SUP冻融精子悬浮在摩德纳的解决方案有或无咖啡因，而没有接受AI母猪，被宰杀晚4小时。子宫中回收的肿瘤坏死因子的子宫的PMN和mRNA表达（定量逆转录聚合酶链反应）的数目的分析IL-6，IL-8，单核细胞趋化蛋白-1和环氧合酶2在子宫内膜。咖啡因降低（P总子宫的PMN和IL-8基因在人工智能后子宫内膜表达式05）两者的数目。IL-8和环氧合酶2 mRNA的AI之后在没有咖啡因的量分别比未接受艾母猪样品升高（P而有肿瘤坏死表达水平治疗之间无差异显著因子妊娠率母猪授精与精子补充有咖啡因（16 23; 70％）趋向性（P总之，除了咖啡因的解冻溶液抑制子宫中性粒细胞迁移的，可能是由于子宫内膜下调IL-8的表达。
S. Yamaguchi[a]; C. Suzuki[b]; M. Noguchi[b][c]; S. Kasa[a]; M. Mori[a]; Y. Isozaki[a]; S. Ueda[a]; H. Funahashi[d]; K. Kikuchi[e]; T. Nagai[f]; K. Yoshioka[b]. Effects of caffeine on sperm characteristics after thawing and inflammatory response in the uterus after artificial insemination with frozen-thawed boar semen[J]. Theriogenology, 2013,79(1): 87–93