摘要
The aim of this study was to evaluate the effects of lyophilized egg yolk extender on ram semen cryopreservation. Ejaculates with a thick consistency, rapid wave motion (3–5 on a 0–5 scale) and >75% initial motility were pooled. Sperm were diluted to final concentration of 1/5 (semen/extender) in lyophilized egg yolk or fresh egg yolk extenders using two-step dilution method. The equilibrated semen was frozen in 0.25 mL straws. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) at three time points: after dilution with extender A, equilibration and post-thaw. The results showed that freezing and thawing procedures (dilution, equilibration and thawing) had negative effects on motility (P < 0.001), plasma membrane integrity (P < 0.001), acrosome integrity (P < 0.001) and DNA integrity (P < 0.001). In the study, there were no significant differences between lyophilized and fresh egg yolk extenders when comparing motility, plasma membrane integrity, acrosome integrity and DNA integrity between groups. In conclusion, lyophilized egg yolk extender provided similar cryoprotective effects with fresh egg yolk extender to cryopreserve ram semen.
摘要译文
这项研究的目的是评估冷冻蛋黄延长剂对公羊精液冷冻保存的影响。射精与厚厚的一致性，快速波动（3-5分钟0-5级）和 75％的初始运动性汇集在一起。使用两步稀释法将精子稀释至冻干蛋黄或新鲜蛋黄增量剂中的1/5（精子/增量剂）的最终浓度。平衡的精液在0℃冷冻。25mL吸管。使用低渗肿胀试验（HOST）评估精子样品的精子活力，质膜功能完整性，在三个时间点使用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）来唱FITC-Pisum sativum凝集素（PSA-FITC）和DNA完整性：在用补充剂A稀释后，平衡和解冻后。结果表明，冻融程序（稀释，平衡和解冻）对运动有负面影响（P 0.001），质膜完整性（P 0.001），顶体完整性（P 0.001）和DNA完整性（P 0.001）。在研究中，当比较组之间的运动性，质膜完整性，顶体完整性和DNA完整性时，冻干蛋黄和新鲜蛋黄延伸体之间没有显着差异。总之，冻干蛋黄延长剂提供了与新鲜蛋黄延长剂类似的冷冻保护作用，以冷冻保存公羊精液。
Selim Alcay; M. Berk Toker; Elif Gokce; Burcu Ustuner; N. Tekin Onder; Hakan Sagirkaya; Zekariya Nur; M. Kemal Soylu. Successful ram semen cryopreservation with lyophilized egg yolk-based extender[J]. Cryobiology, 2015,71(2): 329–333