摘要
Development of ways to block virus transmission by aphids could lead to novel and broad-spectrum means of controlling plant viruses. Viruses in the Luteoviridae enhanced are obligately transmitted by aphids in a persistent manner that requires virion accumulation in the aphid hemocoel. To enter the hemocoel, the virion must bind and traverse the aphid gut epithelium. By screening a phage display library, we identified a 12-residue gut binding peptide (GBP3.1) that binds to the midgut and hindgut of the pea aphid Acyrthosiphon pisum. Binding was confirmed by labeling the aphid gut with a GBP3.1–green fluorescent protein fusion. GBP3.1 reduced uptake of Pea enation mosaic virus (Luteoviridae) from the pea aphid gut into the hemocoel. GBP3.1 also bound to the gut epithelia of the green peach aphid and the soybean aphid. These results suggest a novel strategy for inhibiting plant virus transmission by at least three major aphid pest species.
摘要译文
方法来阻止病毒的传播蚜虫发展可能导致控制植物病毒的新型广谱手段。增强的黄症病毒科病毒是专性蚜虫在持久方式，需要病毒体积累的蚜虫血腔传输。进入血腔，病毒体必须绑定和遍历蚜肠上皮细胞。通过筛选噬菌体展示文库，我们确定了12个残基的肠结合肽（GBP3.1），其结合到豌蚜蚜豌豆的肠和后肠。结合被标记的蚜虫肠道有GBP3.1绿色荧光蛋白融合证实。 GBP3.1从豌豆蚜虫肠道进入血腔减少豌豆enation花叶病毒（黄症病毒科）的摄取。 GBP3.1也势必桃蚜的肠道上皮细胞和大豆蚜虫。这些结果表明了一种新策略由至少有三个主要的蚜虫害虫种类抑制植物病毒传播。
Sijun Liu[a]; S. Sivakumar[a]; Wendy O. Sparks[a][c]; W. Allen Miller[b]; Bryony C. Bonning[a]. A peptide that binds the pea aphid gut impedes entry of Pea enation mosaic virus into the aphid hemocoel[J]. Virology, 2010,401(1): 107–116