摘要
Objective
To determine an optimum frequency of semen collection, so as to improve the efficacy of artificial insemination techniques and selective breeding programmes in duck farming. Methods
Thirty two ejaculates were collected by abdominal massage method from two groups of drakes, with 10 in each, at one day and two day intervals respectively. Routine seminal attributes such as volume, colour, pH, spermatozoa concentration, number of spermatozoa per ejaculate, individual motility, live sperm percentage, total sperm abnormalities and time taken to reduce Methylene blue (MBRT) were studied for comparison of their values in one day and two days of interval of semen collection. Twenty female laying ducks maintained for examining the fertility percent were regularly inseminated with 0.1 mL of neat pooled semen for fertility test. Results
The test of significance indicated a significant decrease (P<0.05) in the time taken to reduce methylene blue, a highly significant increase in the sperm concentration (P<0.01) and number of spermatozoa per ejaculation (P<0.01) and a non significant improvement in the seminal characteristics with respect to semen volume and mass motility was found in the ejaculates of two day interval than that of one day interval collections. Conclusion
Collection of semen at two days intervals is recommended for enhancing fertility in artificial insemination of duck. The methylene-blue reduction test, along with spermatozoa count and initial motility estimates were reported to be better indicator of semen quality.
摘要译文
目的测定精液集合的最佳频率，以便改进在鸭养殖人工授精技术和选择育种方案的功效方法32只精液是由腹部按摩的方法从两组打水漂的收集，每组10只，分别为一天，2天间隔常规精液等属性的量，颜色，pH值，精子浓度，每次射精精子，个别运动性，活精子百分数的数，减少亚甲基蓝（MBRT）采取总精子畸形和时间进行了研究他们在一天和值的比较精液采集间隔两天维持检查生育％的20只雌性产蛋鸭与生育力测试整齐的精液池的0L定期授精结果显着性的试验表明在减少亚甲蓝，在精子浓度高显著增加所用的时间一显著下降（P 0（P 01）和每射精精子（P 数0ND相对于精液的体积和质量蠕动的精液特性的非显著的改善在比一天的时间间隔收集2天区间射精发现建议在duckThe亚甲基蓝还原试验人工授精生育增强结论，二十多天的时间间隔精液的收集，与精子计数沿线和初步估计蠕动据报道，精液质量较好的指标
AK Nahak; SC Giri; DN Mohanty; PC Mishra; SK Dash. Effect of frequency of collection on seminal characteristics of White Pekin duck[J]. Asian Pacific Journal of Reproduction, 2015,4(1): 70–73