摘要
The objective of this experiment was to evaluate the effect of mangosteen peel powder (MSP) on rumen fermentation, nutrient digestibility and microbial population in swamp buffaloes (Bubalus bubalis) fed on a rice straw based diet. MSP is a fruit peel that contains plant secondary compounds (condensed tannins and saponins). Eight, rumen-fistulated 4-year old, male swamp buffaloes with 350±4 kg live weight were randomly assigned to receive two dietary treatments (T1=non-supplementation of MSP and T2=supplementation of MSP at 100 g/(head×day)) according to a t-test design. All animals were fed concentrate mixtures at 0.5% of body weight and rice straw was fed ad libitum for 37 days with the first 30 days for feed adaptation and voluntary feed intake measurement, while the last 7 days were for samples collection. The results revealed that there was no difference between treatments on dry matter intake and nutrient digestibility by MSP supplementation (P>0.05). Furthermore, MSP supplementation did not influence ruminal pH, temperature, ammonia nitrogen and blood urea nitrogen. Interestingly, propionic acid (C3) production was significantly increased by MSP supplementation (P<0.05) while total volatile fatty acids, acetic acid (C2) and butyric acid were similar between treatments. Moreover, C2:C3 ratio was reduced by MSP supplementation. In addition, estimated rumen methane was significantly reduced by MSP supplementation (P<0.05). Application of quantitative PCR to quantify cellulolytic bacteria (16S rRNA) targets revealed that MSP supplementation did not change population of Fibrobacter succinogenes and Ruminococcus albus (P>0.05) while total bacteria population were significantly increased by MSP supplementation. The Ruminococcus flavefaciens and methanogens population were significantly decreased as MSP were supplemented (P<0.05). Nitrogen intake, excretion (fecal and urine) and balance (absorption and retention) were similar between treatments whilst microbial nitrogen supply and efficiency of microbial protein synthesis were increased by MSP supplementation. In conclusion, supplementation of MSP as a source of plant secondary compounds exhibited no negative effect on feed intake, nutrient digestibility, ruminal fermentation characteristics, nutrient utilization and microbial protein synthesis. But rather, it could influence on rumen methanogenic population and hence, possibly mitigate methane production in swamp buffaloes fed on a rice straw based diet.
摘要译文
该实验的目的是评价对瘤胃发酵的山竹果皮粉末（MSP）的效果，养分消化率和微生物类群分布的沼泽水牛（水牛）喂食水稻秸秆为主的饮食习惯。MSP是果皮含有植物次生化合物（缩合单宁和皂苷）。八，瘤胃瘘管的4岁，faloes与350±4公斤活体重随机分配根据t-检验设计成接纳两个日粮处理（T1 \x3d不可补充的MSP和T 2 \x3d补充的MSP以100g /（头×天））。全部动物喂混合精在体重的0.5％和水稻秸秆自由采食37天，前30天为饲料的适应和自由采食量的测量，而过去的7天分别为样本采集。结果显示，有（干物质采食量和消化率由MSP补充治疗间无差异P05）。此外，MSP补充不影响瘤胃pH值，温度，氨氮和血液尿素氮。有趣的是，丙酸（C 3）产量显著（增加了MSP补充P此外，C 2：C 3的比例降低了，MSP补充。此外，估计的瘤胃甲烷由MSP补充显著减少（P定量PCR的pplication量化纤维分解菌（16S rRNA）的目标表明，MSP的补充没有改变Fibrobacter琥珀酸和瘤胃球菌的人口（P0.05），而总人口的细菌均显著增加了MSP的补充。在瘤胃球菌和产甲烷人口是显著下降为MSP进行补充（PL和尿）和平衡（吸收和保留）是微生物，而供氮和微生物蛋白质的合成处理的效率分别提高了MSP补充相似。总之，MSP的补充植物次生物质的来源上展出采食量，消化率，瘤胃发酵特性没有负面影响，养分利用率和微生物蛋白质的合成。而是，它可能对瘤胃产甲烷人口，从而影响，可能减少甲烷生产喂食水稻秸秆为主的饮食习惯沼泽水牛。
M. Wanapat[a]; V. Chanthakhoun[a][b]; K. Phesatcha[a]; S. Kang[a]. Influence of mangosteen peel powder as a source of plant secondary compounds on rumen microorganisms, volatile fatty acids, methane and microbial protein synthesis in swamp buffaloes[J]. Livestock Science, 2014,162: 126–133