摘要
The aims of the study were to determine which of Days 5, 6 or 7 after the blood plasma progesterone concentration (PPC) of bitches first reached 6–9 nmol/L (Day 0) yield the highest fertility and whether day of insemination affects the gender ratio of conceptuses. Six bitches were inseminated on Days 5 and 6 and 6 on Days 6 and 7. Ten million progressively motile frozen-thawed sperm from each of 5 dogs were pooled for the first insemination. The same number of sperm from 5 other dogs were pooled for the second insemination. Only one batch of semen from each dog was used on all bitches, which largely prevented any effect of male and semen. Twenty-three autosomal microsatellites and the amelogenin gene were used to determine the paternity and gender of the conceptuses. Pregnancy rate was 100%. Out of 103 ovulations 66 conceptuses were conceived (conception rate: 64%). The proportion of available oocytes fertilised was 0.11, 0.56, and 0.27 for Days 5, 6, and 7, respectively. The odds of fertilisation was 16.7 and 4.2 times higher from insemination on Day 6 compared to Day 5 (P < 0.001) and Day 7 (P = 0.013), respectively. The numbers of male- and female conceptuses were equal (33 each) and gender was independent of insemination day (P = 0.18). This study suggests that intrauterine insemination of bitches should best be done 6 days after PPC first reaches a value between 6 and 9 nmol/L with a second insemination one day later.
摘要译文
这项研究的目的是确定哪些天5，6或7的母狗的血浆孕酮浓度（PPC）后首次达到6-9 nmol / L的（0天）产量最高生育率和人工授精的一天是否影响conceptuses的性别比例。六母狗被授精上天5和6和6上天6和从各5只狗7.千万逐步能动冻融精子合并为第一授精。5其他狗相同数量的精子汇集第二人工授精。只有一批从每只犬精液被用在所有的母狗，这在很大程度上阻碍男性和精液的任何影响。二十三个常染色体微卫星和釉原蛋白基因被用来确定conceptuses的侍和性别。怀孕率为100％25。出103排卵66 conceptuses被设想（受孕率：64％25）。可用卵母细胞的受精的比重为0.11，0.56，和0.27天5，6和7，分别。受精的机率较高于第6天16.7和4.2倍，从授精相比5日;分别与第7天（p = 0.013），（P所述0.001）。以男性和女性conceptuses的人数相等（33个）和性别是独立的人工授精天（P = 0.18）。这项研究表明，雌性的宫腔内人工授精最好应该做6天，PPC首先到达6和9 nmol / L的之间的值与第二人工授精一天之后。
D. Steckler[a]; J.O. Nöthling[a]; C. Harper[b]. Prediction of the optimal time for insemination using frozen-thawed semen in a multi-sire insemination trial in bitches[J]. Animal Reproduction Science, 2013,142(3-4): 191–197