期刊文献

Effects of Lactic Acid Bacteria and Fermented Milks on Eicosanoid Production by Intestinal Epithelial Cells 收藏

乳酸菌及发酵奶的二十烷生产的影响肠上皮细胞

原文求助发布源

作者

Amanda Fiander[1]; Shannon Bradley[2]; Perry C. Johnson-Green[1] and Julia M. Green-Johnson[1,2,3];

作者单位

1 Author Fiander and Johnson-Green are with School of Nutrition and Dietetics, Acadia Univ., Wolfville, NS, Canada. 2 Authors Bradley and Green-Johnson are with Faculty of Science, Univ. of Ontario Inst. of Technology, Oshawa, ON, Canada. 3 Author Johnson-Green is also with Clavaria Solutions, Oshawa, ON, Canada. Direct inquiries to author Julia Green-Johnson, Faculty of Science Unv. of Ontario Institute of Technology, Oshawa ONL1H ZK4, Canada (Email: julia.green-johnson@uoit.ca)

关键词

probiotic; eicosanoid; Lactobacillus; fermented milk; opiod

关键词译文

益生菌;花生酸;乳杆菌;发酵乳;阿片

发布日期

31 MAY 2006

页码

M81-M86

DOI

10.1111/j.1365-2621.2005.tb07107.x

来源信息

Journal of Food Science ISSN：0022-1147, 2005年, 70卷, 2期, M81-M86页

摘要

Fermented milk products produced with probiotic lactic acid bacteria (LAB) have attracted interest due to their potential health benefits. Probiotic bacteria have a range of immunomodulatory activity, interacting with a variety of cell types in the immune system. Interactions with intestinal epithelial cells (IEC) are an avenue through which probiotics and their fermented milks can influence production of key immunoregulatory molecules, including cytokines and eicosanoids. The eicosanoids, which include the prostaglandins (PGs), are lipid mediators implicated in both acute and chronic inflammatory processes. The primary objective of this study was to determine the ability of probiotic LAB and their ferments to interact with IEC and influence their eicosanoid production. Effects of LAB and their milk ferments on prostaglandin E₂ (PGE₂) and prostaglandin F₂ (PGF₂) production by human IEC lines were determined using a competitive enzyme immunoassay. LAB alone did not alter interleukin (IL)-1β-induced prostaglandin production by IEC. However, milk fermented with Lac-tobacillus (L.) rhamnosus strain R0011 significantly suppressed IL-1β-induced levels of PGE₂ and PGF₂, an effect which was counteracted by the addition of strain R0011. Milk ferments prepared withL. acidophilus strain R0052 were less effective in down-regulation of PG production by IEC. Naltrexone, an opioid receptor antagonist, blocked the suppressive effects of L. rhamnosus R0011 milk ferments on PGF₂ production by IEC, suggesting that the bioactivity the ferments is opioid receptor-mediated. These findings support immunomodulatory potential of fermented food components through interactions with intestinal epithelial cells.

摘要译文

用益生乳酸菌（LAB）生产的发酵乳产品已吸引了兴趣，由于其潜在的健康益处。益生菌有一系列免疫调节活性，用在免疫系统的各种细胞类型的相互作用。互动与肠上皮细胞（IEC）是一种途径，通过它益生菌及其发酵乳可以影响生产的关键免疫调节分子，包括细胞因子和类花生酸类物质。所述类花生酸，其中包括前列腺素（PG的），都牵连在急性和慢性炎症过程脂质介质。本研究的主要目的是确定益生菌LAB和它们发酵，以符合IEC相互作用并影响他们的花生酸类生产的能力。LAB的影响和他们的牛奶发酵的前列腺素E 2（PGE 2）和前列腺素F 2（PGF 2）生产人类IEC线是用竞争酶联免疫测定。LAB本身并不改变白介素（IL）-1诱导水平的PGE 2和PGF 2，将其抵消通过加入应变R0011的效果的。牛奶发酵制成withL。嗜酸乳杆菌菌株r0052的不太有效地下调PG生产由IEC。纳曲酮，阿片样受体拮抗剂阻断L的抑制效果鼠李R0011牛奶发酵的PGF 2的生产通过IEC，表明生物活性的发酵是阿片受体介导的。这些发现支持发酵食品组分通过与肠上皮细胞的相互作用的免疫调节的潜能。

Amanda Fiander[1]; Shannon Bradley[2]; Perry C. Johnson-Green[1] and Julia M. Green-Johnson[1,2,3];. Effects of Lactic Acid Bacteria and Fermented Milks on Eicosanoid Production by Intestinal Epithelial Cells[J]. Journal of Food Science, 2005,70(2): M81-M86