摘要
We have reported that artificial insemination (AI) with frozen-thawed boar semen supplemented with caffeine increased the number of uterine sperm by inhibiting the migration of polymorphonuclear leukocytes (PMNs) into the uterine lumen, thereby improving the fertility of gilts and sows. The objective of the present study was to examine the effects of the addition of the antioxidant beta-mercaptoethanol (bME) and caffeine to the thawing solution on the function of frozen-thawed sperm, on the phagocytic activity of PMNs for sperm, and on the fertility of sows after AI. When frozen-thawed sperm were cultured in the presence of 25 or 50 μm bME, sperm capacitation and spontaneous acrosome reactions were inhibited (P < 0.01). There was no effect of bME on phagocytic activity of PMNs for sperm in vitro. When hormonally treated (400 IU of equine chorionic gonadotropin + 200 IU of human chorionic gonadotropin) weaned sows experienced a single intrauterine insemination with frozen-thawed sperm (25 × 108 sperm per 50 ml dose) 40 h after subsequent hCG administration, pregnancy and farrowing rates were unaffected by the addition of 50 μm bME (pregnancy rate, 20 vs 21% in controls; farrowing rate, 20 vs 21%; n = 15 and 14, respectively). However, litter size tended to be higher than in the presence of 50 μm bME compared to its absence (10.0 ± 1.0 vs 5.7 ± 1.5, respectively; P < 0.07). Thus, the addition of bME to the thawing solution containing caffeine could be of benefit for improving the function of frozen-thawed sperm without influencing the phagocytic activity of PMNs for sperm. Although there were no statistically significant effects of bME on pregnancy or farrowing rates, the litter size tended to be higher in the sows subjected to a fixed-time single AI treatment with synchronized ovulation.
摘要译文
射精（AI）与补充有咖啡因冻融公猪精液增加子宫精子数目通过抑制多形核白细胞（PMN）的迁移入子宫腔，从而提高后备母猪和经产母猪的生育能力。本研究的目的是研究在冻融精子的功能在加入抗氧化剂β-巯基乙醇（BME）和咖啡因的解冻溶液中的效果，在中性粒细胞的精子，并在母猪AI后生育的吞噬活性。当冷冻解冻精子培养于25或50的存在精子获能和顶体自发反应受到抑制（P）的人绒毛膜促性腺激素断奶母猪经历一个宫腔内人工授精与冻融精子（25×10 SUP 8 / SUP每个精子50毫升剂量）随后hCG注射后40小时，怀孕和分娩率不受加入50产仔数趋向于比在50存在下更高加入BME的含有咖啡因的解冻溶液可能是有益的改善冻融精子的功能而不影响中性粒细胞的吞噬活动精子。虽然没有BME对妊娠或分娩率统计显著的影响，产仔数往往是在经受固定时间的单AI治疗同步排卵母猪高。
S. Yamaguchi[a]; H. Funahashi[b]. Effect of the addition of beta-mercaptoethanol to a thawing solution supplemented with caffeine on the function of frozen-thawed boar sperm and on the fertility of sows after artificial insemination[J]. Theriogenology, 2012,77(5): 926–932