摘要
In this study, biological networks were reconstructed from genes and metabolites significantly associated with residual feed intake (RFI) in beef cattle. The networks were then used to identify biological pathways associated with RFI. RFI is a measure of feed efficiency, which is independent of body size and growth; therefore selection for RFI is expected to result in cattle that consume less feed without adverse effects on growth rate and mature size. Although several studies have identified genes associated with RFI, the mechanisms of the biological processes are not well understood. In this study, we utilised the results obtained from two association studies, one using 24 genes and one using plasma metabolites to reconstruct biological networks associated with RFI using IPA software (Igenuity Systems). The results pointed to biological processes such as lipid and steroid biosynthesis, protein and carbohydrate metabolism and regulation of gene expression through DNA transcription, protein stability and degradation. The major canonical pathways included signaling of growth hormone, Oncostatin M, insulin-like growth factor and AMP activated protein kinase, and cholesterol biosynthesis. This study provides information on potential biological mechanisms, and genes and metabolites involved in feed efficiency in beef cattle.
摘要译文
在这项研究中，生物的网络从基因和代谢物与残余采食量（RFI）在肉牛显著相关重建。网络，然后用来确定与射频干扰相关的生物途径。 RFI是饲料效率，这是独立的机构规模和增长的措施;因此，选择的RFI预期导致消耗而对增长率和成熟的尺寸产生不良影响少喂牛。虽然一些研究已经确定了RFI相关的基因，所述生物学过程的机制尚不十分清楚。在这项研究中，我们利用从两个关联研究中获得的结果，一个使用的24个基因和一个采用等离子代谢产物来重建使用IPA软件（Igenuity系统）RFI相关的生物网络。结果指出，通过DNA的转录生物过程，如脂和类固醇的生物合成，蛋白质和碳水化合物的代谢和基因表达的调控，蛋白质稳定性和降解。主要的典型途径包括生长激素信令，制瘤素M，胰岛素样生长因子和AMP活化蛋白激酶，和胆固醇的生物合成。这项研究提供了潜在的生物学机制，以及基因和代谢产物参与了饲料转化效率在肉牛的信息。
Brian Karisa[1]; Stephen Moore[2] and Graham Plastow[1,*];. Analysis of biological networks and biological pathways associated with residual feed intake in beef cattle[J]. Animal Science Journal, 2014,85(4): 374-387