摘要
In detecting chemical properties of food, different molecules and ion channels are involved including members of the acid-sensing ion channels (ASICs) family. Consistently ASICs are present in sensory cells of taste buds of mammals. In the present study the presence of ASICs (ASIC1, ASIC2, ASIC3 and ASIC4) was investigated in the taste buds of adult zebrafish (zASICs) using Western blot and immunohistochemistry. zASIC1 and zASIC3 were regularly absent from taste buds, whereas faint zASIC2 and robust zASIC4 immunoreactivities were detected in sensory cells. Moreover, zASIC2 also immunolabelled nerves supplying taste buds. The present results demonstrate for the first time the presence of zASICs in taste buds of teleosts, with different patterns to that occurring in mammals, probably due to the function of taste buds in aquatic environment and feeding. Nevertheless, the role of zASICs in taste remains to be demonstrated.
摘要译文
在检测食品的化学性质，不同的分子和离子通道参与包括酸敏感离子通道（ ASIC）的家族的成员。一贯的ASIC存在于哺乳动物的味蕾的感觉细胞。在本研究中的ASIC （ ASIC1 ， ASIC2 ， ASIC3和ASIC4 ）的存在进行了研究使用免疫印迹和免疫组化成年斑马鱼（ zASICs ）的味蕾。zASIC1和zASIC3来自味蕾经常缺席，而淡淡的zASIC2和强大的免疫反应zASIC4感官细胞进行检测。此外，还zASIC2神经immunolabelled供应味蕾。本结果表明，首次zASICs的硬骨鱼的味蕾存在下，不同的图案，该发生在哺乳动物中，可能是由于味蕾在水生环境和供给的功能。尽管如此， zASICs的味道的作用仍有待证明。
E. Viña[a][b][1]; V. Parisi[c][1]; R. Cabo[a]; R. Laurà[c]; S. López-Velasco[a]; A. López-Muñiz[a]; O. García-Suárez[a]; A. Germanà[c]; J.A. Vega[a][d]. Acid-sensing ion channels (ASICs) in the taste buds of adult zebrafish[J]. Neuroscience Letters, 2013,536: 35–40