摘要
Laparoscopic artificial insemination has an important role in felid conservation but it is costly and includes surgical risk. Therefore, radiographic contrast medium combined with non-surgical transcervical AI to verify intrauterine gamete placement could be a viable alternative. Gamete-rescued fresh and frozen-thawed sperm were extended with one of two commercial contrast media (nonionic and ionic), with osmolarity adjusted to 320–330 mOsm, or feline optimized culture medium (control). Percent motility, forward progression status, and acrosomal integrity were recorded every 30 min for 4 h. Sperm penetration abilities were assessed by coincubating treated sperm with conspecific in vitro matured oocytes for 18 to 20 h, and presumptive zygotes and embryos were fixed and stained to determine sperm penetration and fertilization rate. There was reduced motility and acrosomal integrity in frozen-thawed versus fresh sperm (P < 0.05). Neither radiographic contrast medium induced adverse effects on fresh sperm motility relative to control medium (P > 0.05), but motility of frozen-thawed sperm decreased when treated with nonionic radiographic contrast medium compared to control medium (P < 0.05). There were no differences in acrosomal integrity between radiographic contrast and control media in fresh (P > 0.05) or frozen sperm (P > 0.05). Neither radiographic contrast media decreased the numbers of morphologically normal sperm (P > 0.05) or reduced the ability of domestic cat sperm to penetrate (P > 0.05) or fertilize (P > 0.05) conspecific oocytes. Ionic radiographic contrast medium can be added to fresh or frozen-thawed domestic cat sperm with no adverse effect on motility, morphology, acrosomal integrity or oocyte penetration rates, and thus may be used to facilitate further development of transcervical AI procedures.
摘要译文
腹腔镜人工授精拥有猫科动物保护的重要作用，但它是昂贵的，包括手术风险。因此，结合非手术宫腔镜AI来验证宫腔配子放置放射造影剂可能是一个可行的选择。配子救出新鲜和冷冻解冻精子进行了扩展与两个商用造影剂（非离子型和离子），用的渗透压1调整为320-330毫渗量，或猫优化培养基（控制）。百分之蠕动，向前进展状态和顶体完整性记录每30分钟4小时。精子穿透能力被coincubating用同种的处理精子在体外成熟的卵母细胞进行18至20小时进行评估，和推定受精卵和胚胎固定和染色，以确定精子穿透和受精率。有减少运动和顶体完整的冻融与鲜精（P无论是X线造影剂诱导的精子活力相对新鲜的不利影响，以控制介质（P但是，当用非离子型射线照相造影处理的培养基相比，控制介质（P冻融精子活力降低有在X线造影和控制媒体之间的顶体完整新鲜无差异（P既不射线照相造影下降形态正常的精子的数目（P05）同种卵母细胞。离子型射线照相造影剂可以被添加到新鲜或冷冻 - 解冻的家猫精子对运动没有不利影响，形态学，顶体完整性或卵母细胞的渗透速率，并且因此可以被用来促进宫颈人工智能程序进一步发展。
S.A. Barnes; A.M. Cepeda; L.M. Penfold. Effects of radiographic contrast media on domestic cat epididymidal sperm[J]. Theriogenology, 2011,75(2): 329–336