摘要
The aim of this study was to investigate the clinical relevance of measuring blood concentrations of serum amyloid A (SAA), haptoglobin (Hp) and fibrinogen (Fib) in horse reproductive management, and changes in response to artificial insemination (AI) with frozen–thawed semen. Standardbred mares (n = 18) with different reproductive status (eight healthy mares in first postpartum oestrus, five healthy barren mares and five mares with endometritis) were inseminated with frozen–thawed semen. Endometritis was evaluated during oestrus by bacteriological culture, cytology and presence of ultrasonically visible intrauterine fluid during oestrus. Concentrations of SAA, Hp and Fib were analysed in the blood in every 48 h during oestrus and until 5, 6 or 7 days after AI. The day of sampling and number of blood samples varied between mares because of length of the oestrus and time of AI. Changes in concentrations of SAA, Hp and Fib were evaluated based on the day of sampling regard to AI and classification of the mares. There were no differences in SAA, Hp and Fib concentrations over time before or after AI or between the groups of mares. The insemination of mares with frozen–thawed semen did not increase the plasma concentrations of SAA, Hp and Fib above clinical threshold concentration and there were no differences between susceptible or healthy mares.
摘要译文
这项研究的目的是调查测量血清淀粉样蛋白A的血药浓度(SAA)在马繁殖管理的临床意义,结合珠蛋白(HP)和纤维蛋白原(FIB),和变化响应于人工授精(AI)的冷冻 - 解冻的精液。 Standardbred母(N \x3d 18)与生殖的不同状态(在产后第一次发情只健康的母马,只健康贫瘠的母马,五马子宫内膜炎)进行授精与冷冻精液解冻。发情期间,通过细菌培养子宫内膜炎评价,细胞学检查和超声可见宫腔液发情期间存在。 SAA,惠普和纤维蛋白原的血液中的浓度每48小时在发情期间,直到5进行了分析,后AI 6或7天。采样和血液样本的数目的一天,因为人工智能的发情和时间的长度的母马之间变化。变化SAA的浓度,基于采样方面的母马以AI和分类的一天马力和纤维蛋白原进行了评价。有在SAA没有差异,惠普和纤维蛋白原浓度超过之前或之后的AI母马的群体之间或时间。母马与冻融精液授精没有增加的SAA的血浆浓度,惠普和纤维蛋白原以上临床阈浓度并有敏感或健康的母马之间没有差异。
U. Tuppits[a]; T. Orro[a]; S. Einarsson[b]; K. Kask[a]; A. Kavak[a]. Influence of the uterine inflammatory response after insemination with frozen–thawed semen on serum concentrations of acute phase proteins in mares[J]. Animal Reproduction Science, 2014,146(3-4): 182–186