摘要
Alkaline phosphatase (AP) was detected using ELF-97® in silicon-starved Pseudo-nitzschia multiseries cells; thus, we tested two, alternative hypotheses: Pseudo-nitzschia multiseries has a high phosphate demand, showing signs of phosphate deficiency even when concentrations of orthophosphate are high, or silicate deficiency can stimulate the AP enzyme in this species. We also studied the effect of silicon deficiency on AP in three other common marine diatoms: Thalassiosira pseudonana, Nitzschia pusilla, and Nitschia closterium. Each of the species tested showed a different pattern of AP regulation. AP levels, however, increased in the four diatoms as a result of silicon deficiency, suggesting that AP may be involved in a variety of intracellular processes related to silicon deficiency. Additionally, the results of this study indicate that AP could be stimulated by stressors other than phosphate deficiency, such as silicon deficiency; therefore, it should be used cautiously as an indicator of phosphate limitation.
摘要译文
用ELF-97碱性磷酸酶（AP）检测另一种假设：拟菱形藻multiseries具有较高的磷酸盐的需求，呈现出磷酸脱氢酶缺乏症的迹象，即使正磷酸盐的浓度高，或硅酸盐缺乏能刺激酶AP该物种。我们也研究了AP硅缺陷的影响在三个其他常见的海洋硅藻：海链pseudonana，菱形pusilla和Nitschia藻。每个测试的物种表现出的AP调节的不同的图案。 AP水平，然而，增加了四个硅藻硅不足的结果，这表明AP可以参与各种与硅缺陷细胞内过程的。此外，这项研究的结果表明，AP可以通过压力比磷酸盐缺乏其他诸如硅缺乏刺激;因此，它应谨慎使用作为磷酸盐限制的指示。
Soledad Fuentes [1] [2] Gary H. Wikfors [1] Shannon Meseck [1]. Silicon Deficiency Induces Alkaline Phosphatase Enzyme Activity in Cultures of Four Marine Diatoms[J]. Estuaries and Coasts, 2014,37(2): 312-324