摘要
The effect of arsenic (25 and 50 μM As for 1 and 5 d) was analysed in wild type (WT) and Arabidopsis thaliana (L.) Heynh plants deficient in NADPH oxidase C (AtrbohC). The content of H2O2 and malondialdehyde (MDA) increased with the As concentration, while the opposite effect was found for NO in WT and AtrbohC plants. The As treatment reduced catalase and increased glutathione reductase activities to the same extent in WT and AtrbohC plants, although the induction of all SOD isoforms (mainly CuZn–SODs) was observed in WT plants, the opposite effects being found in AtrbohC plants. Glycolate oxidase (H2O2 producers) considerably increased with the concentration and time of treatment with As in WT and AtrbohC mutants. Arsenic induced the uptake and translocation of P, S, Cu, Zn, and Fe in WT plants, while in AtrbohC plants the opposite trend was noted and the uptake of As became considerably lower than in WT plants. These results suggest that As causes oxidative stress by inducing glycolate oxidase, while NADPH oxidase does not appear to participate in ROS overproduction but could be critical in regulating antioxidant defences as well as the transport and translocation of As and macro/micronutrients.
摘要译文
砷（25和50的作用的H 2 O 2和丙二醛含量（MDA）随As浓度，而被发现相反的效果对NO在WT和AtrbohC植物。在作为处理降低过氧化氢酶，并在WT和AtrbohC植物增加谷胱甘肽还原酶活性至相同程度，虽然观察到野生的植物SOD的所有亚型的诱导（主要是铜锌-SOD能），被发现在AtrbohC植物相反的效果。乙醇酸氧化酶（H 2 O 2的生成器）显着增加，在WT和AtrbohC突变体的浓度和处理时间为。砷引起的P，S，铜的吸收和转运，锌，和铁中的WT植物，而在AtrbohC植物相反的趋势指出和As的摄取变得比在野生型植物相当低。这些结果表明，如通过诱导乙醇酸氧化酶引起的氧化应激，勒NADPH氧化酶似乎不参与ROS生产过剩但也可以是在调节抗氧化防御以及由于运输和易位和宏/微营养素的关键。
D.K. Gupta[a]; M. Inouhe[b]; M. Rodríguez-Serrano[a]; M.C. Romero-Puertas[a]; L.M. Sandalio[a]. Oxidative stress and arsenic toxicity: Role of NADPH oxidases[J]. Chemosphere, 2013,90(6): 1987–1996