摘要
The objective of this study was to determine the developmental rates and relative abundance of Hsp 70.1 and Glut-1 transcripts in in vivo- and in vitro-produced (IVP) bovine embryos in media supplemented with bovine serum albumin (BSA) or different oestrous cow serum concentrations. In experiment 1, in vitro maturation and culture media were supplemented with 0.4% BSA or 1, 5, 10 or 20% of oestrous cow serum (ECS). The analysis of the expression of Hsp 70.1 and Glut-1 was carried out in individual days 7 and 8 embryos by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay. In experiment 2, in vivo-produced morulae were collected on day 7 of the oestrous cycle and employed for the comparison of the relative abundances of Hsp 70.1 and Glut-1 transcripts with IVP morulae produced using two protein sources (10% ECS or 0.4% BSA). No differences were observed in cleavage rate among groups, but blastocyst formation (27%) and hatching rates (78%) were significantly higher in IVP embryos produced with 20% ECS than the other groups (p < 0.05). No significant differences were observed in the relative abundances of Hsp 70.1 and Glut-1 mRNA in days 7 and 8 blastocysts expanded blastocysts between groups. The abundances of mRNA for those genes were similar between IVP and in vivo-produced morulae. In spite of the alterations observed in embryonic development, the presence of serum at distinct concentrations did not appear to alter the relative abundance profiles of Hsp 70.1 and Glut-1 compared with controls or the BSA supplementation to the IVP media.
摘要译文
这项研究的目的是确定的发育率及热休克蛋白70的相对丰度。1和转运蛋白1转录在接近体内并在补充有牛血清白蛋白（BSA）或不同发情牛血清浓度媒体体外生产（IVP）牛胚胎。在实验1，体外成熟和培养基补充有0.4％BSA或1，5，10或发情牛血清（ECS）的20％。热休克蛋白70的表达分析。1和转运蛋白1进行在个别天7和8的胚胎通过半定量逆转录聚合酶链反应（RT-PCR）方法。在实验2中，体内生产的桑椹胚上收集的发情周期的第7天，并用于热休克蛋白70的相对丰度的比较。生产采用两种蛋白质来源（10％ECS或0.4％BSA）1和转运蛋白1的转录与IVP桑椹胚。无差异观察卵裂率组之间，但胚泡形成（27％）和孵化率（78％）为显著高于生产用20％ECS比其他组的IVP胚胎（对无显著差异观察的Hsp 70.1和转运蛋白1 mRNA的相对丰度在天7和8囊胚扩大组之间囊胚。mRNA的那些基因的丰度分别为IVP之间以及在生物体内可产生桑椹胚相似。尽管在胚胎发育中观察到的改变的，血清中的不同浓度的存在并未改变的Hsp 70.1的相对丰度概况和转运蛋白1与对照或牛血清白蛋白补充的IVP媒体相比较。
ATD de Oliveira[1,2]; RFF Lopes[2] and JL Rodrigues[1];. Gene Expression and Developmental Competence of Bovine Embryos Produced In Vitro with Different Serum Concentrations[J]. Reproduction in Domestic Animals, 2006,41(2): 129-136