期刊文献

Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study 收藏

反对瘦素和生长素的作用在马黄体调节：体外研究
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作者
António Galvão;[1;2] Angela Tramontano;[3] Maria Rosa Rebordão;[1] Ana Amaral;[1] Pedro Pinto Bravo;[1] Anna Szóstek;[2] Dariusz Skarzynski;[2] Antonio Mollo;[3] and Graça Ferreira-Dias[1]
作者单位
[1]C.I.I.S.A., Faculty of Veterinary Medicine, University of Lisbon, Portugal [2]Institute of Animal Reproduction and Food Research of PAS, Olsztyn, Poland [3]Department of Animal Medicine, Production and Health (MAPS), Faculty of Veterinary Medicine, University of Padua, Viale dell’Università 16, 35020 Legnaro, Padua, Italy
页码
13
DOI
10.1155/2014/682193
来源信息
Mediators of Inflammation ISSN：0962-9351, 2014年, 13页
摘要
Metabolic hormones have been associated with reproductive function modulation. Thus, the aim of this study was: (i) to characterize the immunolocalization, mRNA and protein levels of leptin (LEP), Ghrelin (GHR) and respective receptors LEPR and Ghr-R1A, throughout luteal phase; and (ii) to evaluate the role of LEP and GHR on progesterone (P4), prostaglandin (PG) E2 and PGF2α, nitric oxide (nitrite), tumor necrosis factor-α (TNF); macrophage migration inhibitory factor (MIF) secretion, and on angiogenic activity (BAEC proliferation), in equine corpus luteum (CL) from early and mid-luteal stages. LEPR expression was decreased in late CL, while GHR/Ghr-R1A system was increased in the same stage. Regarding secretory activity, GHR decreased P4 in early CL, but increased PGF2α, nitrite and TNF in mid CL. Conversely, LEP increased P4, PGE2, angiogenic activity, MIF, TNF and nitrite during early CL, in a dose-dependent manner. The in vitro effect of LEP on secretory activity was reverted by GHR, when both factors acted together. The present results evidence the presence of LEP and GHR systems in the equine CL. Moreover, we suggest that LEP and GHR play opposing roles in equine CL regulation, with LEP supporting luteal establishment and GHR promoting luteal regression. Finally, a dose-dependent luteotrophic effect of LEP was demonstrated
摘要译文
代谢激素已与生殖功能modulationThus相关联，本研究的目的是：（ⅰ）来表征免疫定位，mRNA和瘦素蛋白水平（LEP），生长素（GHR）和各自的受体LEPR和GHR-R1A，整个黄体期;和（ii）以评估对孕酮（P 4），前列腺素（PG）电子2和PGF2α的LEP和GHR的作用，一氧化氮（亚硝酸盐），肿瘤坏死因子α（TNF）;巨噬细胞迁移抑制因子（MIF）的分泌，并在血管生成活性（BAEC增殖），在从早期和中期黄体stagesLEPR表达马黄体（CL）在CL末期被降低，而GHR / GHR-R1A系统中相同的stageRegarding分泌活性增加，GHR在CL年初下降P 4，但增加PGF 2伪，亚硝酸盐和TNF在CLConversely中旬，LEP增加P 4，PGE 2，血管生成活性，MIF，TNF和亚硝酸盐CL月初，在分泌活性LEP的剂量依赖性mannerThe体外作用通过GHR恢复，当这两个因素共同作用目前的结果证明了马CLMoreover LEP和GHR系统的存在，我们认为LEP和GHR在马CL调节中发挥相反的作用，与LEP支承黄体建立和GHR促进黄体regressionFinally，LEP的剂量依赖性促黄体效应证实
António Galvão;[1;2] Angela Tramontano;[3] Maria Rosa Rebordão;[1] Ana Amaral;[1] Pedro Pinto Bravo;[1] Anna Szóstek;[2] Dariusz Skarzynski;[2] Antonio Mollo;[3] and Graça Ferreira-Dias[1]. Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study[J]. Mediators of Inflammation, 2014: 13