摘要
Viral, bacterial, parasitic and fungal pathogens pose a significant, current threat to global biodiversity. A virulent fungal pathogen (Pseudogymnoascus destructans; Pd) emerged in hibernating bats in eastern North America in 2006. In this paper, we seek to inform epidemiological models of the progression of Pd into populations of the little brown bat (Myotis lucifugus) in central Canada by characterizing the spatial genetic structure of the host ahead of the imminent arrival of Pd. We sampled 242 bats from eight hibernacula spanning 92,623 km2 and two ecozones. We genotyped all individuals at eight microsatellite loci and sequenced 300 bp of HVII in a subset (n = 72) to test the null hypothesis of contemporary panmixia. We found evidence of spatial genetic structure associated with ecozone boundaries, and a predominant north–west to south–east directionality of bat movements among hibernacula, which opposes the current approach of the pathogen. Our large study area (larger than the dispersal distance of individual bats) allowed us to detect the first evidence of contemporary population structure among hibernacula of M. lucifugus. Our results suggest that the potential spread of Pd into north-central Canada may be retarded by the opposing direction of gene flow of the host species, and our findings of directional gene flow can be used to inform management strategies for the spread of Pd into the area. KeywordsWhite nose syndrome Little brown bat Central North America Spatial genetic structure Hibernacula Ecozones
摘要译文
病毒，细菌，寄生虫和真菌病原体构成显著，电流威胁全球biodiversityA毒性真菌病原体（Pseudogymnoascus destructans;PD）出现在冬眠蝙蝠在北美东部的2006In本文中，钯进展到小棕蝠（鼠耳蝠lucifugus）在加拿大中部的人口第钯即将提前到来特征的主机空间遗传结构我们取样的242蝙蝠来自八个hibernacula跨越92623公里SUP 2 / SUP和两个生态区我们所有的基因型个体在八个微卫星位点进行测序300 bp的HVII的一个子集（N \x3d 72）来测试当代随机交配的零假设我们发现与生态带的边界相关的空间遗传结构的证据，以及主导西北部击球动作hibernacula之间东南方向性，相对向的病原体的当前做法我们的大型研究区域（不是单个蝙蝠的扩散距离较大）使我们能够检测Mlucifugus的hibernacula间当代人口结构的第一个证据我们的研究结果表明，潜在的Pd扩散到北部和中部加拿大可以通过宿主物种的基因流动的相对方向被延迟，而我们的定向基因流的研究结果可以用来告知管理战略钯蔓延入区KeywordsWhite鼻综合症小棕蝠北美中部的空间遗传结构Hibernacula生态区
Christina M. Davy [1] [2] Felix Martinez-Nunez [1] Craig K. R. Willis [1] Sara V. Good [1]. Spatial genetic structure among bat hibernacula along the leading edge of a rapidly spreading pathogen[J]. Conservation Genetics, 2015,16(5): 1013-1024