期刊文献

IDENTIFICATION OF THE TOXIC DINOFLAGELLATES ALEXANDRIUM CATENELLA AND A. TAMARENSE (DINOPHYCEAE) USING DNA PROBES AND WHOLE-CELL HYBRIDIZATION1 收藏

使用DNA探针和全细胞杂交 1 使用DNA探针和A.Tamarense（Dinophyceae）鉴定毒性的Dinoflageles alexandrium catenella和A. tamarense（Dinophyceae）
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作者
Masao Adachi[1]; Yoshihiko Sako[2,‡] and Yuzaburo Ishida[2];
作者单位
1 Laboratory of Aquatic Environmental Science, Department of Aquaculture, Faculty of Agriculture, Kochi University, Otsu-200, Monobe, Nankoku, Kochi 783, Japan 2 Laboratory of Marine Microbiology, Department of Applied Bioscience, Graduate School of Agriculture, Kyoto University, Kyoto 606-01, Japan
关键词
Alexandrium; identification; in situ hybridization; ITS; Pyrrophyta; species-specific DNA probe
关键词译文
亚历山大;鉴别;原位杂交;它的;助剂;特异性DNA探针
发布日期
28 JUN 2008
页码
1049-1052
DOI
10.1111/j.0022-3646.1996.01049.x
来源信息
Journal of Phycology ISSN：0022-3646, 1996年, 32卷, 6期, 1049-1052页
摘要
Fluorescent DNA probes (cCAT-F1 and cTAM-Fl) complementary to the 3′ end of ribosomal RNA (rRNA) internal transcribed spacer 1 sequences (ITS 1: positions 154–176) of toxic species of Alexandrium catenella (Whedon and Kofoid) Taylor and A. tamarense (Lebour) Taylor were applied to various cultures of the genus Alexandrium and several other phytoplankters using whole-cell fluorescent in situ hybridization. cCAT-F1 and cTAM-F1 reacted with targeted strains of A. catenella (catenella type) and A. tamarense (tamarense type), respectively, and did not react with isolates of A. affine (Inoue et Fukuyo) Balech, A. fraterculus (Balech) Balech, A. insuetum Balech, A. lusitanicum Balech, A. pseudogonyaulux (Biecheler)Horiguchi ex Yuki et Fukuyo comb. nov., nor isolates of Prorocentrum micans Ehrenberg, Amphidinium carterae Hulburt, Heterocapsa triquetra (Ehrenberg) Stein, Gymnodinium mikimotoi Miyake et Kominami ex Oda, Skeletonema costatum (Greville) Cleve, Heterosigma akashiwo (Hada) Hada, and Chattonella antiqua (Hada) Ono. DNase I and RNase A treatment showed that probes hybridized to ribosomal DNA, not rRNA. Probes were localized at the bottom of the U-shaped nucleus, a region that corresponds to the nucleolus. The probes are highly specific for particular strains of A. catenella and A. tamarense and are applicable for identifying these species collected from cultured and possibly natural populations.
摘要译文
荧光DNA探针（CCAT-F1和CTAM-F1）的互补的核糖体RNA的3端（rRNA）的内转录间隔序列1（ITS 1：位置154-176）链状亚历山大藻的有毒物种（温登和科福伊德）泰勒和A.藻（Lebour）泰勒施加属亚历山大使用全细胞荧光原位杂交各种文化和其它几个浮游植物。CCAT-F1和CTAM-F1以A catenella（catenella型）和塔玛亚历山大藻（藻型），有针对性的菌株分别反应，并没有与甲仿射分离反应（Inoue等Fukuyo）Balech，A fraterculus（Balech）Balech，A insuetum Balech，A lusitanicum Balech，A pseudogonyaulux（Biecheler）堀口前徐怀钰等Fukuyo梳理。十一月，也没有隔离海洋原甲藻藻，Amphidinium carterae Hulburt，Heterocapsa竹叶（藻）斯坦因，裸甲藻Miyake等小南前小田，中肋骨条藻（格雷维尔）克利夫，赤潮异弯藻（哈达）哈达，和海洋卡盾蝇（哈达）小野。抗酶和RNA酶的处理表明，探针杂交到核糖体DNA，而不是rRNA基因。探针定位在U形核的底部，对应于核仁的区域。该探测器是非常具体的A. catenella和塔玛亚历山大藻的特殊菌株，并适用于确定这些种养殖和可能的自然种群收集。
Masao Adachi[1]; Yoshihiko Sako[2,‡] and Yuzaburo Ishida[2];. IDENTIFICATION OF THE TOXIC DINOFLAGELLATES ALEXANDRIUM CATENELLA AND A. TAMARENSE (DINOPHYCEAE) USING DNA PROBES AND WHOLE-CELL HYBRIDIZATION1[J]. Journal of Phycology, 1996,32(6): 1049-1052