摘要
Generally, laparoscopic artificial insemination (LAI) provides a higher success rate than of cervical insemination in goats. However, the sperm distribution after LAI in goats remains unknown, particularly when frozen-thawed semen is used. This study evaluated the distribution of frozen-thawed goat spermatozoa after LAI and compared the effects of sperm numbers and deposition sites (unilateral and bilateral sites) on pregnancy rate. In experiment 1, the frozen-thawed spermatozoa were stained either with CellTracker Green CMFDA (CT-Green) or CellTracker Red CMPTX (CT-Red), and in vitro evaluations of viability and motility were performed. In experiment 2, the labeled spermatozoa were deposited via LAI into the left (CT-Green) and right (CT-Red) uterine horns (n = 4). After ovariohysterectomy (6 hours after insemination), the distributions of green- and red-colored spermatozoa were assessed via tissue section, flushing, and the oviductal contents were also collected. Experiment 3 was designed to test the pregnancy rates in a group of 120 does after LAI using different numbers of spermatozoa (60 and 120 × 106 sperm per LAI) and different deposition sites. The results demonstrated that the fluorochromes used in this study did not impair sperm motility or viability. Frozen-thawed goat spermatozoa can migrate transuterinally after LAI, as evidenced by the observations of both CT-Green– and CT-Red–labeled spermatozoa in both uterine horns. Lower numbers of spermatozoa (60 × 106) that are inseminated unilaterally (either ipsilateral or contralateral to the site of ovulation) can efficiently be used for LAI in goats (with a 56.67% pregnancy rate).
摘要译文
一般来说，腹腔镜人工授精（LAI）提供了一个更高的成功率比山羊颈椎授精。然而，在山羊LAI后精子的分布尚不清楚，特别是当冷冻 - 解冻的精液被使用。该研究评估了冻融精子山羊分布LAI后精子相比数量和怀孕率沉积部位（单侧和双侧网站）的影响。在实验1中，冻融精子染色或者用CellTracker CMFDA绿（CT-绿）或CellTracker红CMPTX（CT-红），在生存能力和活力的体外评价进行。在实验2中，标记的精子通过LAI存入左侧（CT-绿色）和右（CT-红色）子宫角（N \x3d 4）。卵巢子宫切除术（受精后6小时）后，绿色和红色的精子的分布通过组织切片进行了评估，潮红，并且还收集了输卵管内容。实验3的目的是测试的怀孕率在一组120并使用不同数量的精子后LAI（60和120×10 SUP 6 / SUP每LAI精子）和不同的沉积位点。结果表明，在本研究中使用的荧光染料不损害精子活力或生存力。冻融精子山羊可以LAI后transuterinally迁移，就证明了这两种CT-格陵兰和观察两子宫角CT-红色标记的精子。较低的精子数（60×10 SUP 6 / SUP）被单方面人工授精（无论同侧或对侧排卵的网站），可有效地用于LAI山羊（带56。67％的怀孕率）。
Nitira Anakkul[a]; Junpen Suwimonteerabutr[a]; Theerawat Tharasanit[a]; Sarawanee Khunmanee[b]; Paweena Diloksumpan[b]; Debra K. Berg[c]; Mongkol Techakumphu[a]. Sperm distribution and fertilization after unilateral and bilateral laparoscopic artificial insemination with frozen-thawed goat semen[J]. Theriogenology, 2014,82(8): 1137–1144