摘要
The baculovirus-insect cell expression system is a well-established technology for the production of heterologous viral (glyco)proteins in cultured cells, applicable for basic scientific research as well as for the development and production of vaccines and diagnostics. Arboviruses form an emerging group of medically important viral pathogens that are transmitted to humans and animals via arthropod vectors, mostly mosquitoes, ticks or midges. Few arboviral vaccines are currently available, but there is a growing need for safe and effective vaccines against some highly pathogenic arboviruses such as Chikungunya, dengue, West Nile, Rift Valley fever and Bluetongue viruses. This comprehensive review discusses the biology and current state of the art in vaccine development for arboviruses belonging to the families Togaviridae, Flaviviridae, Bunyaviridae and Reoviridae and the potential of the baculovirus-insect cell expression system for vaccine antigen production The members of three of these four arbovirus families have enveloped virions and display immunodominant glycoproteins with a complex structure at their surface. Baculovirus expression of viral antigens often leads to correctly folded and processed (glyco)proteins able to induce protective immunity in animal models and humans. As arboviruses occupy a unique position in the virosphere in that they also actively replicate in arthropod cells, the baculovirus-insect cell expression system is well suited to produce arboviral proteins with correct folding and post-translational processing. The opportunities for recombinant baculoviruses to aid in the development of safe and effective subunit and virus-like particle vaccines against arboviral diseases are discussed.
摘要译文
杆状病毒 - 昆虫细胞表达系统是一个成熟的技术，用于生产在培养细胞中的异源病毒（糖）蛋白，适用于基础科学研究以及对疫苗和诊断的开发和生产。虫媒病毒形成一个新兴的组被通过节肢动物载体中，大多是蚊子，蜱或蠓传染给人类和动物医学上重要的病毒病原体。少数虫媒病毒疫苗是目前可用的，但对于对一些高致病性虫媒病毒安全有效的疫苗，如基孔肯雅，登革热，西尼罗河一个不断增长的需求，裂谷热和蓝舌病病毒。这一全面的审查讨论的属于披膜病毒科虫媒病毒的技术开发疫苗的生物学和目前的状态，黄病毒科在系统疫苗抗原产生3这四个虫媒病毒家族的成员都具有包封的病毒体，并具有复杂的结构，在其表面上显示免疫糖蛋白。病毒抗原的杆状病毒表达往往导致正确折叠和加工（糖）蛋白能够诱导在动物模型和人类中的保护性免疫。如虫媒病毒占据，在所述virosphere一个独特的位置，他们还积极复制在节肢动物细胞，杆状病毒 - 昆虫细胞表达系统是非常适合于生产虫媒病毒蛋白质的正确折叠和翻译后加工。对于重组杆状病毒以在安全和有效的亚单位和病毒样颗粒的疫苗针对虫媒疾病的发展有助于机会进行了讨论。
Stefan W. Metz; Gorben P. Pijlman. Arbovirus vaccines; opportunities for the baculovirus-insect cell expression system[J]. Journal of Invertebrate Pathology, 2011,107(Sup): S16–S30