摘要
We compared the effects of extenders of frozen-thawed semen on post-thaw sperm characteristics and the distribution of frozen-thawed spermatozoa in the female genital tract after fixed-timed deep intrauterine insemination (DIUI) in sows. Frozen semen samples were thawed and diluted in either modified Modena solution (mMS) or porcine fertilization medium (PFM) containing theophylline, adenosine and cysteine. Sperm quality, assessed in vitro based on motility using a computer-assisted sperm analyzer and the integrity of the plasma and acrosomal membranes using flow cytometry, was evaluated at 0.5, 1.5, 3 and 6 h after thawing. Progressive motility and the percentage of spermatozoa with damaged acrosomal membranes in PFM were significantly better than in mMS throughout the 6 h. Sows with estrus synchronized using prostaglandin F2 alpha, equine chorionic gonadotropin and human chorionic gonadotropin (hCG) were inseminated once with mMS- or PFM-diluted 5 × 108 frozen-thawed spermatozoa by DIUI at 34 h after the hCG injection. At 4 h after DIUI, reproductive tracts were recovered from 30 sows. There were significantly fewer polymorphonuclear leukocytes (PMNs) and more spermatozoa outside PMNs in the uterine horn after PFM treatment than with mMS. When 22 sows were administered DIUI with 10 × 108 frozen-thawed spermatozoa at 36 h after hCG, the pregnancy rates did not differ significantly between the mMS- (36%) and PFM- (64%) treated groups. Thus, PFM enhanced progressive sperm motility but increased sperm membrane damage compared with mMS; it also suppressed the migration of PMNs into the uterine lumen.
摘要译文
母猪在解冻后精子特征冻融精液和冻融精子在女性生殖道的固定定时的深宫腔内人工授精（DIUI）之后的分布冷冻精液样品解冻，并在任一改性摩德纳溶液（MMS）或猪受精培养基（PFM）含有茶碱，腺苷和cysteineSperm质量稀释，在基于使用计算机辅助精子分析仪和使用流式细胞术等离子体和顶体膜的完整性蠕动体外评估，在01进行评价thawingProgressive活力和精子在PFM损坏顶体膜上的百分比后3〜6小时是在整个6小时比彩信显著更好与发情母猪使用前列腺素F 2阿尔法同步，马绒毛膜促性腺激素和人绒毛膜促性腺激素（hCG）与mMS-或人工授精一次PFM-稀释5脳10 SUP 8 / SUP解冻绒毛膜促性腺激素注射后34 H精子通过DIUI在DIUI 4小时后，生殖道，从30头母猪回收有显著减少多形核白细胞（PMN），更精子外中性粒细胞在子宫角PFM治疗比MMS后当22母猪给予DIUI用10×10 SUP 8 / SUP解冻的绒毛膜促性腺激素后36小时精子，怀孕率没有的mMS-（36％25）和PFM-（64％25）处理groupsThus之间显著不同，PFM逐步增强精子活力，但与彩信相比增加精子细胞膜损伤;它也被抑制中性粒细胞的迁移进入子宫腔
Michiko Noguchi[a][1]; Koji Yoshioka[a]; Hirokazu Hikono[a]; Chie Suzuki[a]; Kazuhiro Kikuchi[b]. Effect of semen extenders on frozen-thawed boar sperm characteristics and distribution in the female genital tract after deep intrauterine insemination in sows[J]. Animal Reproduction Science, 2015,163: 164–171