摘要
The effects of representative surfactants on the growth of Saccharomyces cerevisiae WSH-J701 and glutathione (GSH) production were studied. The anionic surfactant sodium dedecyl sulfate (SDS) and the cationic surfactant cetyltrimethyl ammonium bromide (CTAB) had critical inhibitory concentrations of 0.5 and 0.025 g/l, respectively, to cell growth during cultivation. Above these concentrations, cell growth was greatly inhibited or even ceased. Nonionic surfactants such as polyoxyethylene lauryl ether (Brij30) and polyoxyethylene sorbitan monooleate (Tween80) could also affect cell growth only when their concentrations reached much higher levels. Under the critical concentration, SDS and CTAB increased the extracellular GSH concentration by 10 and 15%, but above this level, they improved extracellular GSH concentration by 35 and 60%, respectively. Otherwise, total GSH concentration and intracellular GSH content decreased slightly. The effect of Brij30 was somewhat like ionic surfactants, extracellular GSH concentration could be increased by 50% but the intracellular GSH content was reduced by 33%. Tween80 seemed to have no effect on extracellular GSH accumulation, but total GSH concentration and intracellular GSH content decreased gradually by increasing its concentration.
摘要译文
关于酿酒酵母WSH-J701和谷胱甘肽（GSH）生产的增长代表表面活性剂的影响进行了研究。所述阴离子表面活性剂dedecyl硫酸钠（SDS）和阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）的临界抑制浓度为0.5和0.025克/升，分别在培养过程中对细胞的生长。上述这些浓度，细胞生长抑制极大，甚至停止。ONIC表面活性剂如聚氧乙烯月桂醚（Brij30）和聚氧乙烯脱水山梨糖醇单油酸酯（吐温80）也可能影响细胞的生长，只有当它们的浓度达到高得多的水平。下的临界浓度，SDS和CTAB增加细胞外谷胱甘肽浓度由10和15％，但此水平之上，它们由35和60％的改进的细胞外谷胱甘肽浓度分别。否则，总GSH产量和胞内GSH含量略有下降。 Brij30的效果有点像离子表面活性剂，细胞外谷胱甘肽浓度可提高50％，但在细胞内GSH含量降低了33％。吐温80似乎对细胞外谷胱甘肽积累没有影响，但总的谷胱甘肽浓度和细胞内GSH含量通过增加其浓度逐渐下降。
Gongyuan Wei[a]; Yin Li[a][b]; Guocheng Du[a]; Jian Chen[a][b]. Effect of surfactants on extracellular accumulation of glutathione by Saccharomyces cerevisiae[J]. Process Biochemistry, 2003,38(8): 1133–1138