摘要
The influence of Toxoplasma gondii on semen variables and sperm morphology of sheep was evaluated in eight reproductive males distributed into three experimental groups: GI, three sheep inoculated with 2.0 × 105 of P strain oocytes; GII, three sheep infected with 1.0 × 106 of RH strain tachyzoites and; GIII two control sheep. Clinical (rectal temperature, cardiac and respiratory frequencies), parasite and serology exams (IIF) were realized. Sperm variables (volume, motility, vigor and concentration) and semen morphology for each sheep were also evaluated. Thus, semen and blood collections were assessed on post-inoculation days (PIDs)-1,3,5,7,11,14 and weekly thereafter up to PID 70. Clinical alterations were observed (hypothermia and anorexia) in infected sheep from groups GI and GII. Parasitic outbreaks were detected in five sheep. All the infected sheep produced antibodies against T. gondii from PID 5 onwards, reaching a peak of 4096 and 8192 for group GI and GII sheep, respectively. Differences (P < 0.05) were observed regarding the ejaculate volume between the inoculated groups (oocytes and tachyzoites) and control. Even though experimental toxoplasmic infection resulted in clinical symptomology in the inoculated sheep, the minimal alterations in sperm pathologies could not be directly attributed to T. gondii.
摘要译文
弓形虫对精液变量和羊的精子形态的影响在八个男性生殖评价分配到三个实验组：GI，三羊接种2.0×10 SUP 5 / SUP点应变的卵母细胞; GII，三羊感染了1.0×10 SUP 6 / SUP RH株速殖子和; GIII两个控制羊。临床（肛温，心脏和呼吸频率），寄生虫和血清学检查（IIF）的实现。精子变量（体积，蠕动，活力和浓度）和精液形态为每只羊也进行了评估。因此，精液和血液采集进行了评估在接种后日（PID）的-1,3,5,7,11，14周后达70 PID改建临床从组GI和GII观察（低温和厌食）在受感染的羊。在五只羊进行检测寄生虫的爆发。所有受感染的羊生产免受PID 5起弓形虫抗体，达到4096和8192组的GI和GII羊，分别高峰。差异（P05）观察有关接种组（卵母细胞和速殖子）和控制的射精量。尽管实验弓形虫感染后的临床症候在接种羊，改建精子病理学最小不能直接归因于弓形虫。
W.D.Z. Lopes[a]; A.J. Costa[a]; F.A. Souza[a]; J.D.F. Rodrigues[a]; G.H.N. Costa[a]; V.E. Soares[a]; G.S. Silva[b]. Semen variables of sheep (Ovis aries) experimentally infected with Toxoplasma gondii[J]. Animal Reproduction Science, 2009,111(2-4): 312–319