摘要
The developed method for simultaneous detection of aflatoxin B1 (AFB1) and aflD genes can effectively monitor from the source and reduce the safety problems and economic losses caused by the production of aflatoxin, which can be of great significance for food safety regulations. In this paper, we constructed a sensitive and convenient fluorescent biosensor to detect AFB1 and aflD genes simultaneously based on fluorescence resonance energy transfer (FRET) between quantum dots (QDs) and a black hole quenching agent. A stable “Y” shaped aptasensor was employed as the detection platform and a double quantum dot labeled DNA fragment was utilized to be the sensing element in this work. When the targets of AFB1 and aflD genes were presented in the solution, the aptamer in the “Y” shaped probe is specifically recognized by the target. At this time, both Si-carbon quantum dots (Si-CDs) and CdTe QDs are far away from the BHQ1 and BHQ3 to recover the fluorescence. The linear range of the prepared fluorescence simultaneous detection method was as wide as 0.5–500 ng·mL−1 with detection lines of 0.64 ng·mL−1 for AFB1 and 0.5–500 nM with detection lines of 0.75 nM for aflD genes (3σ/k). This fabricated fluorescent biosensor was further validated in real rice flour and corn flour samples, which also achieved good results. The recoveries were calculated by comparing the known and found amounts of AFB1 which ranged from 88.4 to approximately 115.32% in the rice flour samples and 90.7 ~ 102.58% in the corn flour samples. The recoveries of aflD genes ranged from 84.32 to approximately 109.3% in the rice flour samples and 89.48 ~ 100.99% in the corn flour samples. Therefore, the proposed biosensor can significantly improve food safety and quality control through a simple, fast, and sensitive agricultural product monitoring and detection system.Graphical abstract
摘要译文
开发的用于同时检测黄曲霉毒素B1（AFB1）和AFLD基因的方法可以有效地监测来源，并减少由于产生黄曲霉毒素而造成的安全问题和经济损失，这对食品安全法规可能具有重要意义。在本文中，我们构建了一个敏感且方便的荧光生物传感器，以基于量子点（QD）和黑洞淬灭剂之间的荧光共振能量转移（FRET）同时检测AFB1和AFLD基因。使用稳定的“ Y”形恰感语作为检测平台，并利用双量子点标记为DNA片段的双量子点是这项工作中的传感元素。当在溶液中介绍AFB1和AFLD基因的靶标时，靶标在“ Y”形探针中的适体。目前，Si-碳量子点（SI-CD）和CDTE QD都远离BHQ1和BHQ3，以恢复荧光。制备的荧光同时检测方法的线性范围宽高达0.5-500 ng·Ml -1 ，检测线为0.64 ng·ml -1 -1 ，用于AFB1和0.5–500 nm的检测线为0.75 nm的AFLD基因（3σ/K）。这种制造的荧光生物传感器在真实的米粉和玉米粉样品中得到了进一步验证，这也取得了良好的效果。通过比较米粉样品中的已知和发现的AFB1量和90.7〜102.58％的范围为115.32％，可以计算回收率。米粉样品中AFLD基因的回收率范围从84.32到约109.3％，玉米粉样品中的回收率为89.48〜100.99％。因此，提出的生物传感器可以通过简单，快速和敏感的农产品监测和检测系统显着改善食品安全和质量控制。
Li; Yaqi[1];Sun; Qingyue[2];Chen; Xin[1];Peng; Shuangfeng[1];Kong; Dezhao[1];Liu; Chang[1];Zhang; Qi[1];Shi; Qiaoqiao[1];Chen; Yong[3]. Simultaneous detection of AFB1 and aflD gene by “Y” shaped aptamer fluorescent biosensor based on double quantum dots[J]. Analytical and Bioanalytical Chemistry, 2024,416(4): 883-893