期刊文献

Participation of WD repeat-containing protein 54 (WDR54) in rat sperm-oocyte fusion through interaction with both IZUMO1 and JUNO 收藏

通过与Izumo1和Juno的相互作用，WD重复蛋白54（WDR54）参与大鼠精子融合

原文求助发布源

作者

Wanjin Xing [a]

作者单位

[a]Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, School of Life Sciences, Inner Mongolia University, Hohhot, PR ChinaInner Mongolia Key Laboratory for Molecular Regulation of the Cell, School of Life Sciences, Inner Mongolia University, Hohhot, PR China[b]Inner Mongolia Comprehensive Center for Disease Control and Prevention, Hohhot, PR ChinaInner Mongolia Comprehensive Center for Disease Control and Prevention, Hohhot, PR China[c]Inner Mongolia Key Laboratory of Molecular Pathology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, PR ChinaInner Mongolia Key Laboratory of Molecular Pathology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, PR China

发布日期

15 January 2024

页码

286-297

DOI

10.1016/j.theriogenology.2023.10.031

来源信息

Theriogenology ISSN：0093-691X, 2024年, 214卷, 286-297页

摘要

Fertilization is a complex process that depends on the fusion of the cell membrane of sperm with that of oocyte, and it involves sperm-oocyte recognition, binding, and fusion, which are mediated by multiple proteins. Among those proteins, IZUMO1 and its receptor JUNO have been identified as essential factors for sperm-oocyte recognition and fusion. However, the interaction between IZUMO1 and JUNO alone does not lead to cell membrane fusion, suggesting the involvement of additional proteins in sperm-oocyte membrane fusion. In this study, we have discovered that a protein called WDR54, which consists of WD-repeat modules, is located on the cell membrane of sperm, as well as on the cell membrane and in the cytoplasm of the oocyte. We have found that WDR54 is involved in sperm-oocyte fertilization. When sperm and oocyte were treated with anti-WDR54 ascites, the in vitro fertilization (IVF) rate significantly decreased. Furthermore, our research has shown that WDR54 interacts with both IZUMO1 and JUNO, and it colocalizes with IZUMO1 on the surface of the sperm head and with JUNO on the oocyte surface. Through structural analysis of the putative complexes of WDR54-IZUMO1 and WDR54-JUNO, we infer that these three proteins could form a complex of JUNO-WDR54-IZUMO1-JUNO (referred to as the “JWIJ complex”) on the oocyte surface. Our findings suggest that WDR54 is an important factor involved in sperm-oocyte adhesion and fusion. This discovery provides new insight into the mechanisms of mammalian sperm-oocyte adhesion and fusion.

摘要译文

受精是一个复杂的过程，取决于精子与卵母细胞的细胞膜的融合，并且涉及由多种蛋白质介导的精子识别，结合和融合。在这些蛋白质中，Izumo1及其受体Juno已被确定为精子识别和融合的基本因素。但是，仅Izumo1和Juno之间的相互作用并不会导致细胞膜融合，这表明其他蛋白质参与了精子 - 绿色素膜融合。在这项研究中，我们发现一种称为WDR54的蛋白质，该蛋白质由WD重复模块组成，位于精子的细胞膜以及细胞膜和卵母细胞的细胞质上。我们发现WDR54参与了精子 - 素质受精。当用抗WDR54腹水处理精子和卵母细胞时，体外受精（IVF）率显着降低。此外，我们的研究表明，WDR54与Izumo1和Juno相互作用，并且它与精子头表面的Izumo1共定位，并在卵母细胞表面与Juno共定位。通过对WDR54-izumo1和WDR54-JUNO的假定复合物的结构分析，我们推断出这三种蛋白可以形成卵母细胞表面上的Juno-WDR54-izumo1-Juno（称为“ Jwij Complex”）的Juno-Wdr54-izumo1-Juno（称为“ Jwij Complex”）。我们的发现表明，WDR54是精子粘附和融合涉及的重要因素。这一发现为哺乳动物精子粘附和融合的机制提供了新的见解。

Wanjin Xing [a]. Participation of WD repeat-containing protein 54 (WDR54) in rat sperm-oocyte fusion through interaction with both IZUMO1 and JUNO[J]. Theriogenology, 2024,214: 286-297