摘要
Potassium diformate (KDF) is a kind of formate, which possesses the advantages of antimicrobial activity, growth promotion and preventing diarrhea in weaned piglets. However, the researches of KDF in animal production mostly focused on apparent indexes such as growth performance and the mechanisms of KDF on intestinal health have not been reported. Thus, porcine small intestinal epithelial cells (IPEC-J2) infected with Enterohemorrhagic Escherichia coli (EHEC) was used to investigate the role of KDF on alleviating intestinal inflammation in this study. The 0.125 mg/mL KDF treated IPEC-J2 cells for 6 h and IPEC-J2 cells challenged with 5 × 107 CFU/mL EHEC for 4 h were confirmed as the optimum concentration and time for the following experiment. The subsequent experiment was divided into four groups: control group (CON), EHEC group, KDF group, KDF+EHEC group. The results showed that KDF increased the cell viability and the gene expression levels of SGLT3 and TGF-β, while decreased the content of IL-1β compared with the CON group. The cell viability and the gene expressions of SGLT1, SGLT3, GLUT2, Claudin-1, Occludin and TGF-β, and the protein expression of ZO-1 in EHEC group were lower than those in CON group, whereas the gene expressions of IL-1β, TNF, IL-8 and TLR4, and the level of phosphorylation NF-кB protein were increased. Pretreatment with KDF reduced the content of IgM and IL-1β, the gene expressions of IL-1β, TNF, IL-8 and TLR4 and the level of phosphorylation NF-кB protein, and increased the gene expression of TGF-β and the protein expression of Occludin in IPEC-J2 cells infected EHEC. In conclusion, 0.125 mg/mL KDF on IPEC-J2 cells for 6 h had the beneficial effects on ameliorating the intestinal inflammation because of reduced pro-inflammatory cytokines and enhanced anti-inflammatory cytokines through regulating NF-кB signaling pathway under the EHEC challenge.
摘要译文
钾扩散（KDF）是一种甲酸盐，具有抗菌活性，生长促进和预防断奶小猪腹泻的优势。但是，尚未报道KDF在动物生产中的研究主要集中在明显的索引上，例如增长性能和KDF在肠道健康方面的机制。因此，使用猪肠上皮细胞（IPEC-J2）感染了肠肠肠癌大肠杆菌（EHEC），以研究KDF在这项研究中KDF对减轻肠道炎症的作用。0.125 mg/ml KDF处理过的IPEC-J2细胞6小时，IPEC-J2细胞受到5×10 7 7 cfu/ml EHEC的挑战4 h，确认为以下的最佳浓度和时间实验。随后的实验分为四组：对照组（CON），EHEC组，KDF组，KDF+EHEC组。结果表明，KDF增加了SGLT3和TGF-β的细胞活力和基因表达水平，而与CON组相比，IL-1β的含量降低。SGLT1，SGLT3，Glut2，Claudin-1，occludin和TGF-β的细胞活力和基因表达，EHEC组中ZO-1的蛋白表达低于CON组的蛋白1β，TNF，IL-8和TLR4以及磷酸化NF-μb蛋白的水平增加。用KDF预处理可降低IgM和IL-1β的含量，IL-1β，TNF，IL-8和TLR4的基因表达以及磷酸化NF-list蛋白的水平，并增加了TGF-β的基因表达以及蛋白质的基因表达occludin在感染EHEC的IPEC-J2细胞中的表达。总之，由于促炎细胞因子减少，在EHEC挑战下调节NF-limb信号传导途径，因此，IPEC-J2细胞上的0.125 mg/ml kDF对改善肠道炎症具有有益作用。
Yu-Yun Gao [a]. Potassium diformate alleviated inflammation of IPEC-J2 cells infected with EHEC[J]. Veterinary Microbiology, 2024,291: 110013