期刊文献

A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors 收藏

保持大肠杆菌为杆状病毒表达载体的产生有缺陷的病毒基因组
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作者
Yeon Ho Je [1] Jin Hee Chang [1] Jae Young Choi [1] Jong Yul Roh [1] Byung Rae Jin [2] David R. O'Reilly [3] Seok Kwon Kang [1]
作者单位
1. School of Agricultural Biotechnology, Seoul National University, 103 Seodoon-Dong, Suwon, 441-744, Korea 2. College of Natural Resources and Life Science, Dong-A University, Pusan, 604-714, Korea 3. Department of Biology, Imperial College of Science, Technology and Medicine, London, SW7 2AZ, UK
关键词
AcMNPV ;bacmid ;baculovirus ;expression vector ;miniF replicon
关键词译文
AcMNPV的;杆状病毒质粒;杆状病毒表达载体; miniF复制子
页码
575-582
DOI
10.1023/A:1010301404445
来源信息
Biotechnology Letters ISSN：0141-5492, 2001年, 23卷, 8期, 575-582页
摘要
A novel baculovirus genome has been generated that can be maintained in Escherichia coli and which facilitates the rapid and efficient generation of recombinant baculovirus expression vectors. This genome, designated bAcGOZA, lacks a portion of the essential ORF1629 gene but includes a miniF replicon and selectable kanamycin-resistance marker. The bAcGOZA can replicate only in E. coli and can be rescued by recombination with a transfer vector containing an intact ORF1629. There is no background of non-recombinant virus.
摘要译文
一种新颖的杆状病毒基因组已被产生，可以保持在大肠杆菌和这有利于快速和有效地产生的重组杆状病毒表达载体。这个基因组中，指定bAcGOZA，缺乏必需ORF1629基因的一部分，但它包括一个miniF复制子和可选择的卡那霉素抗性标记。该bAcGOZA可以在大肠杆菌中仅复制，并且可以通过重组用含有一个完整的ORF1629的转移载体救出。没有背景非重组病毒。
Yeon Ho Je [1] Jin Hee Chang [1] Jae Young Choi [1] Jong Yul Roh [1] Byung Rae Jin [2] David R. O'Reilly [3] Seok Kwon Kang [1]. A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors[J]. Biotechnology Letters, 2001,23(8): 575-582