摘要
Endoscopic-assisted transcervical inseminations (TCIs) have become increasingly popular. The aim of this retrospective clinical study was to evaluate data from the TCIs performed at our facility. We evaluated data from January 2018 through December 2021. This included 137 cases with fresh, 67 cases chilled, and 63 cases using frozen-thawed semen. All bitches underwent breeding management to determine the ideal breeding period. All semen samples were evaluated for total number of sperm, total motility, and progressive motility. Pregnancy was determined by B-mode ultrasonography about 4 weeks after the breeding. Litter size was determined by radiographs performed around the last week of gestation. The pregnancy rate was 83.21% for fresh, 67.16% for chilled, and 66.67% for frozen-thawed semen. There was a significant difference in litter size between fresh semen (6.82 puppies per litter) and both chilled (5.21 puppies per litter) and frozen-thawed (4.59 puppies per litter) semen (P < .05). There was no significant difference in litter size between chilled and frozen-thawed semen. There was no difference in pregnancy rates between clinicians performing the inseminations. Pregnancy rate was not different when sedation was used for the insemination (66.67%) compared to when sedation was not used (74.84%; P > .05). Performing 2 TCIs during the fertile period, regardless of the semen type, resulted in an increase of 6.6% in pregnancy rate (P > .05) and an increase of 0.7 puppies per litter, on average (P > .05). These results can be used to help guide recommendations for breeding clients on the best options to increase both pregnancy rate and litter size for their breeding.
摘要译文
内窥镜辅助的透明仪（TCI）变得越来越流行。这项回顾性临床研究的目的是评估我们设施中执行的TCI的数据。我们从2018年1月到2021年12月评估了数据。其中包括137例新鲜，67例冷藏和63例使用冷冻透视精液的病例。所有bit子均经过育种管理，以确定理想的繁殖期。评估所有精液样品的精子总数，总运动和进行性运动。通过B模式超声检查确定妊娠大约在繁殖后4周。垃圾大小由妊娠最后一周左右进行的X光片确定。新鲜的妊娠率为83.21％，冷冻的妊娠率为67.16％，冷冻透视精液的妊娠率为66.67％。新鲜精液（每窝幼犬6.82幼犬）和冷藏（每窝幼犬5.21幼犬）和冷冻透射（每窝4.59幼犬）精液（p <.05）之间存在显着差异。冷冻和冷冻透视精液之间的垃圾大小没有显着差异。执行临床医生的临床医生之间的怀孕率没有差异。与未使用镇静相比，使用镇静作用（66.67％）时，妊娠率没有差异（74.84％； p> .05）。无论精液类型如何，在肥沃期间进行2次TCI，导致妊娠率增加6.6％（p> .05），平均每窝增加0.7只幼犬，平均增加了（p> .05）。这些结果可用于帮助指导建议客户的建议，以提高妊娠率和窝点的繁殖量的最佳选择。
Marco A. Coutinho da Silva [a]. Factors Affecting Pregnancy Success in the Bitch Following Transcervical Insemination[J]. Topics in Companion Animal Medicine, 2023,55: 100800