摘要
Purpose : We investigated whether the human oocyte maturity at the removal of cumulus/corona cells affects the embryo outcome in vitro.
Methods : A total of 620 oocytes, which subsequently underwent blastocyst culture, were included in this analysis. Oocytes that were in prophase or Metaphase I of meiosis at the removal of cumulus/corona cells were in Group II. Oocytes that were in Metaphase II at the removal of cumulus/corona cells were in Group I.
Results : Group I oocytes yielded the highest fertilization rates (96.3% vs. 77.1%, P < 0.001). The incidence of Group II oocytes developing to the blastocyst stage was significantly less than from Group I oocytes (38.1% vs. 86.1%, P < 0.001). The percentage of top-scoring blastocysts from Group I oocytes was higher than that of Group II oocytes (95.4% vs. 76.2%, P < 0.001).
Conclusions : Oocyte maturity at the removal of cumulus/corona cells needs to be considered in selecting good quality blastocysts for embryo transfer.
摘要译文
目的：我们研究了人类卵母细胞成熟的卵丘去除/电晕细胞是否影响体外胚胎的结果。方法：共620卵母细胞，随后进行囊胚培养，被列入这一分析。这是在前期还是减数分裂中期我在去除卵丘/电晕细胞的卵母细胞在第二组。这是在中期II的卵丘去除/电晕细胞的卵母细胞中的一组结果：I组的卵母细胞产生最高的受精率（96.3％对77.1％，PII组卵母细胞发展至胚泡期的发生率显著小于从组I的卵母细胞（38.1％对86.1％，P从第I组卵母细胞得分最高的囊胚的百分比明显高于组II的卵母细胞（95.4％对76.2％，P的高结论：成熟卵母细胞的卵丘去除/电晕细胞需要在选择质量好的囊胚进行胚胎移植加以考虑。
Fu-Jen Huang [1] [2] Hsuan-Wei Huang [1] Kuo-Chung Lan [1] Fu-Tsai Kung [1] Yi-Chi Lin [1] Hsueh-Wen Chang [3] Shiuh-Young Chang [1] [2]. The Maturity of Human Cumulus-Free Oocytes Is Positively Related to Blastocyst Development and Viability[J]. Journal of Assisted Reproduction and Genetics, 2002,19(12): 555-560