期刊文献

Expression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus) 收藏

izumo1和juno在家猫（Felis Catus）中的表达

原文求助发布源

作者

Kaywalee Chatdarong [a]

作者单位

[a]Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, ThailandDepartment of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand[b]Veterinary Pharmacology and Stem Cell Research Laboratory, Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, ThailandVeterinary Pharmacology and Stem Cell Research Laboratory, Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science,Chulalongkorn University, Bangkok 10330, Thailand[c]Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, ThailandVeterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand[d]Department of Veterinary Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, ThailandDepartment of Veterinary Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand[e]Department of Human Anatomy and Psychobiology, IMIB-Pascual Partilla Institute, University of Murcia, 30120, Murcia, SpainDepartment of Human Anatomy and Psychobiology, IMIB-Pascual Partilla Institute, University of Murcia, 30120 Murcia, Spain[f]Department of Cell Biology and Histology, Faculty of Medicine, Campus de Ciencias de La Salud, University of Murcia, Campus Mare Nostrum (CMN), 30120, Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, Campus de Ciencias de la Salud, University of Murcia, Campus Mare Nostrum (CMN), 30120 Murcia, Spain[g]Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de La Salud, 30120, Murcia, SpainInstituto Murciano de Investigacion Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, 30120 Murcia, Spain

发布日期

1 May 2024

页码

70-76

DOI

10.1016/j.theriogenology.2024.02.032

来源信息

Theriogenology ISSN：0093-691X, 2024年, 220卷, 70-76页

摘要

Because of the time-consuming nature of surgical neutering and the rapid rate of reproduction among domestic cats, it is crucial to investigate alternative, nonsurgical methods of contraception for this species. Sperm protein IZUMO1 and its oocyte receptor JUNO have been proposed as potential targets for nonsurgical contraceptives. This study aimed to demonstrate (1) the protein coding sequence of feline IZUMO1 and JUNO, (2) gene expression in specific organs by measuring mRNA levels in different visceral tissues, and (3) the expression of IZUMO1 and JUNO during sperm maturation and folliculogenesis, respectively. Amplification for sequencing of feline IZUMO1 and JUNO was performed using the RT-PCR method. Levels of gene expression in different tissues were evaluated using real-time PCR. In situ hybridization was performed to localize JUNO mRNA in ovarian tissues. The complete coding sequences of IZUMO1 and JUNO were obtained and analyzed. A comparison between protein orthologs demonstrated the conservation of IZUMO1 and JUNO in Felidae. The real-time PCR results from various visceral organs indicated that IZUMO1 was significantly higher in the testis than in other organs, whereas JUNO was significantly higher in the ovary than in other organs. Expression of IZUMO1 was found to be higher in the testes than in the caput, corpus, and cauda of epididymides. In situ hybridization revealed that JUNO mRNA was in the ooplasm and nucleus of the primordial, primary, secondary, and antral follicles. Importantly, this was the first study to demonstrate the IZUMO1 and JUNO genes in the testis and ovary of cats. The results are useful for future research related to these genes and for developing contraceptives against these targets.

摘要译文

由于手术绝育的时间耗时，而家猫之间的繁殖速度迅速，因此至关重要的是研究该物种的替代性非手术避孕方法。精子蛋白Izumo1及其卵母细胞受体Juno已被提议作为非手术避孕药的潜在靶标。这项研究旨在证明（1）通过测量不同内脏组织中的mRNA水平，以及（3）精子成熟和卵泡发生过程中izumo1和Juno的表达，（2）特定器官中的蛋白质编码序列（2）特定器官中的基因表达，分别。使用RT-PCR方法进行了猫Izumo1和Juno测序的放大。使用实时PCR评估了不同组织中基因表达水平。进行原位杂交以将朱诺mRNA定位在卵巢组织中。获得并分析了Izumo1和Juno的完整编码序列。蛋白质直系同源物之间的比较证明了菲利迪科中的Izumo1和Juno的保护。来自各种内脏器官的实时PCR结果表明，睾丸中的Izumo1明显高于其他器官，而卵巢中朱诺的卵巢明显高于其他器官。在睾丸中发现Izumo1的表达高于附子酰胺的Caput，cauda和cauda。原位杂交表明，Juno mRNA在原始，原发性，次级和肛门卵泡的卵子和核中。重要的是，这是第一项证明猫和卵巢中Izumo1和Juno基因的研究。该结果对于与这些基因有关的未来研究以及针对这些靶标的避孕剂很有用。

Kaywalee Chatdarong [a]. Expression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus)[J]. Theriogenology, 2024,220: 70-76