摘要
This study investigated the appropriate way of dietary Acer truncatum leaves (ATL) addition, the effect of disease prevention and its mechanism of action. In experiment 1, 192 Arbor Acres broilers were assigned to 4 treatment groups, fed with basal diets containing 2% bran, replacing it with primary and fermented ATL, and additional 0.3% ATL extract to the basal diet for 42 d, respectively. In experiment 2, 144 broilers were assigned to 3 treatment groups for 21-d trial: (1) C-N group, basal diets, and injected with 0.9% (w/v) sterile saline; (2) C-L group, basal diets, and injected with lipopolysaccharide (LPS); (3) T-L group, ATL diets and injected with LPS. In experiment 1, ATL significantly decreased the index of abdominal fat at 42 d (P < 0.05). ATL extract had a better ability to improve antioxidant capacity and reduce inflammatory levels among all treatment groups, which significantly decreased the content of MDA in the liver and ileum mucosa at 21 d, and increased the expression of IL-10 and Occludin in jejunal mucosa at 42 d (P < 0.05). In experiment 2, ATL significantly increased the level of T-AOC in the liver, decreased the expression of NF-κB in the jejunal mucosa and ileum mucosa (P < 0.05), and restored LPS-induced the changed level of CAT in jejunal mucosa, the expression of IL-6, Claudin-1, and ZO-1 in jejunal mucosa and IL-1β in ileum mucosa (P < 0.05). Analysis of gut microbiota indicated that ATL enhanced the abundances of Bacteroidota and reduced the proportion of Firmicutes (P < 0.05), and the changed levels of T-AOC in body, IL-1β, IL-6, IL-10, and NF-κB in jejunum mucosa and propionic acid in cecal were associated with gut microbiota. Collectively, our data showed that the extract of ATL had a better antioxidant and anti-inflammatory effects than primality and fermented. Extraction of ATL modulated intestinal microbiota, and had a protective effect on oxidative stress, inflammation, and intestinal barrier function in broilers challenged with LPS.
摘要译文
这项研究调查了饮食中的牙齿肌叶（ATL）的适当方法，预防疾病的作用及其作用机理。在实验1中，将192个植物肉鸡分配给4个治疗组，并用含有2％麸皮的基础饮食喂食，用原发性ATL和发酵的ATL代替，并分别为基础饮食添加0.3％的ATL提取物42 d。在实验2中，将144个肉鸡分配到3个治疗组进行21-D试验：（1）C-N组，基础饮食，并注射0.9％（w/v）无菌盐水；（2）C-L组，基础饮食，并注入脂多糖（LPS）；（3）T-L组，ATL饮食，并注入LPS。在实验1中，ATL在42 d时显着降低了腹部脂肪的指数（P <0.05）。ATL提取物具有改善抗氧化能力并降低所有治疗组之间的炎症水平的能力，这大大降低了21 d时肝脏和回肠粘膜中MDA的含量，并增加了IL-10和occludin在空肠粘膜中的表达42 d（p <0.05）。在实验2中，ATL显着提高了肝脏中T-AOC的水平，降低了空肠粘膜和回肠粘膜中NF-κB的表达（p <0.05），并恢复了LPS，并恢复了LPS的变化。，在回肠粘膜中的空肠粘膜和IL-1β中IL-6，Claudin-1和ZO-1的表达（p <0.05）。对肠道微生物群的分析表明，ATL增强了杆菌的丰富性并降低了Firmicutes的比例（P <0.05），并且体内T-AOC的变化水平变化，IL-1β，IL-6，IL-10和NF- NF-盲肠中的空肠粘膜中的κB与肠道菌酸中的丙酸有关。总的来说，我们的数据表明，ATL的提取物具有比原始性和发酵效率更好的抗氧化和抗炎作用。提取ATL调节肠菌群的提取，并对受LPS挑战的肉鸡中的氧化应激，炎症和肠屏障功能具有保护作用。
Xin Yang * [†]. Acer truncatum leaves extract modulates gut microbiota, improves antioxidant capacity, and alleviates lipopolysaccharide-induced inflammation in broilers[J]. Poultry Science, 2023,102(10): 102951