摘要
The supplementation of antimicrobial growth promoters (AGPs) has been banned in many countries because of the emergence of antimicrobial-resistant pathogens in poultry products and the environment. Probiotics have been broadly studied and demonstrated as a promising AGP substitute. Our study is centred on the effects of a multi-strain Bacillus-based probiotic product on broiler production performance and gut microbial profile in a dexamethasone-induced leaky gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)). The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota were studied by 16S rRNA gene sequencing. The Bacillus probiotic product significantly improved production performance and altered caecal gut microbiota (p ≤ 0.05), but no significant impact on microbiota was observed in other gut sections.
摘要译文
由于禽类产品和环境中出现了抗菌病原体，因此在许多国家禁止补充抗菌增长促进剂（AGP）。益生菌已被广泛研究并证明是有希望的AGP替代品。我们的研究集中在多杆菌益生菌产品对肉鸡生产性能和肠道微生物谱的影响上，在地塞米松引起的漏水肠道挑战中。孵化了256只肉鸡小鸡并随机分为四组（小麦大豆粉基底饮食（BD）=不补充的对照（c），BD在第4周（CD）中补充了dexamethasone，BD包含益生菌从第一天（P）和BD，从第一天开始就含有益生菌，并在挑战第4周（PD）中补充了地塞米松。通过16S rRNA基因测序研究了生产性能和凯卡尔，吉萨尔，空肠腔和空肠粘膜拭子微生物群。芽孢杆菌产物显着改善了生产性能并改变了盲肠微生物群（p≤0.05），但在其他肠道切片中未观察到对微生物群的显着影响。
Darwin Horyanto;Yadav S. Bajagai;Advait Kayal;Juhani von Hellens;Xiaojing Chen;Thi Thu Hao Van;Anita RadovanovićDragana Stanley. Bacillus amyloliquefaciens Probiotics Mix Supplementation in a Broiler Leaky Gut Model[J]. Microorganisms, 2024,12(2): 419