摘要
The effects of dietary probiotic supplementation with viable Bacillus subtilis and Bacillus amyloliquefaciens spores on sow performance, immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning were investigated. Ninety-six sows reared in a continuous farrowing system for one full cycle were fed gestation diets during the first 90 d of pregnancy and lactation diets until the end of lactation. The sows were fed a basal diet without probiotics (control; n = 48) or a diet supplemented with viable spores (1.1 × 109 CFU/kg of feed) (probiotic; n = 48). At 7 d of age, sucking piglets (n = 12/group) were provided prestarter creep feed until weaning at 28 d of age. The piglets in the probiotic group were supplemented with the same probiotic and dosage as their dams. Blood and colostrum collected from sows and ileal tissues collected from piglets on the day of weaning were used for analyses. Probiotics increased the weight of piglets (P = 0.077), improved the weaning weight (P = 0.039) and increased both the total creep feed consumption (P = 0.027) and litter gain (P = 0.011). Probiotics also improved the faecal score in the second (P = 0.013) week of life. The immunoglobulin G (IgG) concentrations in sow blood at farrowing and the IgM concentrations in piglet blood at weaning were higher in the probiotic group than in the control group (P = 0.046). The piglets from the probiotic-treated sows showed a higher IgM concentration in the ileal mucosa (P = 0.050) and a lower IgG concentration in the ileal mucosa (P = 0.021) compared with the piglets from control sows. The probiotic-treated piglets had a thicker ileal mucosa (P = 0.012) due to the presence of longer villi and larger Peyer's patches (P < 0.001). B. subtilis and B. amyloliquefaciens were detected in the probiotic-treated piglets but not the control piglets; these bacteria were present in the digesta and villus structures and formed structures resembling biofilms. Overall, Bacillus-based probiotic supplementation improves the health indices of sows and their piglets.
摘要译文
研究了饮食益生菌补充枯草芽孢杆菌和淀粉菌孢子孢子孢子孢子孢子菌对猪的性能，免疫，肠道功能状态和生物膜形成，并研究了益生菌在断奶时的小猪中。在妊娠的前90天和哺乳期饮食中，妊娠饮食喂养妊娠饮食，直到泌乳结束。饲喂母猪的基础饮食，没有益生菌（对照； n = 48）或补充有生存的孢子的饮食（1.1×10 9 9 cfu/kg饲料的饲料）（益生菌； n = 48）。在7 d年龄的时候，提供了吮吸小猪（n = 12/组），直到在28 d时断奶为止。益生菌中的小猪被补充了与大坝相同的益生菌和剂量。在断奶当天，从猪和回肠组织收集的血液和初乳被用于分析。益生菌增加了小猪的重量（P = 0.077），提高了断奶的重量（P = 0.039），并增加了总蠕变饲料消耗量（P = 0.027）和垃圾增益（P = 0.011）。益生菌还改善了生命的第二个（p = 0.013）周的粪便得分。益生菌组的sow血液中母猪血液中的免疫球蛋白G（IgG）浓度高于对照组（p = 0.046）。与对照母猪的小猪相比，来自益生菌处理的母猪的小猪在回肠粘膜中显示出较高的IgM浓度（P = 0.050），而卵形粘膜中的IgG浓度较低（P = 0.021）。由于存在较长的绒毛和较大的Peyer斑块，益生菌处理的小猪具有较厚的回肠粘膜（P = 0.012）（P <0.001）。在益生菌治疗的小猪中检测到枯草芽孢杆菌和链球菌法，但未检测到对照小猪。这些细菌存在于摘要和绒毛结构中，并形成类似于生物膜的结构。总体而言，基于芽孢杆菌的益生菌补充剂可改善母猪及其小猪的健康指数。
Paweł Konieczka; Karolina Ferenc; Jens N. Jørgensen; Lea H.B. Hansen; ... Krzysztof Lipiński. Feeding Bacillus-based probiotics to gestating and lactating sows is an efficient method for improving immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning[J]. Animal Nutrition, 2023,13: 361-372