期刊文献

Photoprotective Effects of Two New Morin-Schiff Base Derivatives on UVB-Irradiated HaCaT Cells 收藏

两个新的Morin-Schiff碱衍生物对UVB辐照的HACAT细胞的光保护作用

原文求助发布源

作者

Sara García-Gil;Azahara Rodríguez-Luna;Javier ávila-Román;Gabriela Rodríguez-García;Rosa E. del Río;Virginia Motilva;Mario A. Gómez-Hurtado;Elena Talero

作者单位

[1] Department of Pharmacology, Faculty of Pharmacy, Universidad de Sevilla, 41012 Seville, Spain [2] Faculty of Health Sciences, Universidad Loyola Andalucía, 41704 Seville, Spain [3] Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, Morelia 58030, Michoacán, Mexico [*] Authors to whom correspondence should be addressed. [†] These authors contributed equally to this work. Antioxidants 2024, 13(1), 134; https://doi.org/10.3390/antiox13010134

关键词

photoprotection; biological filter; UV protection; visprotection; morin; antioxidant; Schiff bases

关键词译文

光保护；生物过滤器；紫外线保护；验证；莫林抗氧化剂；希夫基地

发布日期

2024-01-22

页码

134

DOI

10.3390/antiox13010134

来源信息

Antioxidants ISSN：2076-3921, 2024年, 13卷, 1期, 134页

摘要

Ultraviolet (UV) radiation harms the skin, causing oxidative damage, inflammation, and disruption of the skin’s barrier function. There is considerable interest in identifying new natural ingredients with antioxidant and anti-inflammatory properties to serve as adjuvants in sunscreens. The flavonoid morin (1) can undergo structural modifications to enhance its biological properties. The aim of this study was to synthesize two new morin-Schiff base derivatives, morin oxime (2) and morin semicarbazone (3), comparing their photoprotective effects with that of the parent compound on UVB-exposed HaCaT keratinocytes. The chemical structure of the novel compounds was revealed based on spectroscopic data analysis. Our findings demonstrated that derivatives 2 and 3 enhanced the light absorption capability in the UV–visible (vis) range compared to 1. Tested compounds exhibited a higher scavenger capacity than Trolox. Moreover, pre-treatment with all compounds protected HaCaT cells from UVB-induced cell death. Compound 3 demonstrated the strongest antioxidant effect, reducing reactive oxygen species (ROS) generation and, subsequently, malondialdehyde (MDA) levels. Additionally, compounds 2 and 3 exhibited greater anti-inflammatory effects than compound 1, significantly reducing interleukin (IL)-6 production levels at all tested concentrations. These findings have demonstrated, for the first time, a promising photoprotective activity of two new Schiff base derivatives and suggest their use as natural sunscreen ingredients.

摘要译文

紫外线（UV）辐射会损害皮肤，从而导致氧化损伤，炎症和皮肤屏障功能的破坏。识别具有抗氧化剂和抗炎特性的新天然成分具有很大的兴趣，可以用作防晒霜的佐剂。类黄酮（1）可以进行结构修饰以增强其生物学特性。这项研究的目的是合成两个新的Morin-Schiff碱基衍生物Morin oxime（2）和Morin emicarbazone（3），将它们的光保护作用与母体化合物对UVB暴露的HACAT HACAT角膜生素类细胞的光保护作用进行了比较。基于光谱数据分析揭示了新型化合物的化学结构。我们的发现表明，衍生物2和3增强了紫外线可见度（VIS）范围的光吸收能力，而测试化合物的清除能力比Trolox更高。此外，与所有化合物的预处理均保护了HACAT细胞免受UVB诱导的细胞死亡的影响。化合物3证明了最强的抗氧化作用，降低了活性氧（ROS）的产生，随后是丙二醛（MDA）水平。此外，与化合物1相比，化合物2和3具有更大的抗炎作用，在所有测试浓度下都显着降低了白介素（IL）-6的生产水平。这些发现首次证明了两个新的Schiff碱衍生物的有希望的光保护活性，并提出它们用作天然防晒成分。

Sara García-Gil;Azahara Rodríguez-Luna;Javier ávila-Román;Gabriela Rodríguez-García;Rosa E. del Río;Virginia Motilva;Mario A. Gómez-Hurtado;Elena Talero. Photoprotective Effects of Two New Morin-Schiff Base Derivatives on UVB-Irradiated HaCaT Cells[J]. Antioxidants, 2024,13(1): 134