摘要
SRC gene encodes scavenger receptor class C, a member of the scavenger receptor family, and has only been identified and investigated in invertebrates. Our previous studies have revealed that SRC is a novel candidate gene associated with body weight in Pacific white shrimp (Litopenaeus vannamei). In order to comprehend the underlying mechanism by which LvSRC affects shrimp growth, we analyzed the structure, phylogeny, expression profiles and RNA interference (RNAi) of this gene in L. vannamei. We found that LvSRC contains two CCP domains and one MAM domain, with the highest expression level in the heart and relatively low expression level in other tissues. Notably, LvSRC exhibited significantly higher expression levels in the fast-growing group among groups with different growth rates, suggesting its potential involvement as a gene contributing to the growth of L. vannamei. RNAi of LvSRC inhibited body length and body weight gain compared to the control groups. Moreover, through RNA-seq analysis, we identified 598 differentially expressed genes (DEGs), including genes associated with growth, immunity, protein processing and modification, signal transduction, lipid synthesis and metabolism. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed significant changes in the signaling pathways related to growth, lipid metabolism and immune response, suggesting that LvSRC exhibits the potential to participate in diverse physiological processes and immune regulation. These findings deepen our understanding of the structure and function of the SRC in shrimp and lay the foundation for further research into the regulatory mechanism of LvSRC. Additionally, they provide potential applications in shrimp genetics and breeding.
摘要译文
SRC基因编码清道夫受体家族成员C类C级C类，仅在无脊椎动物中被鉴定和研究。我们以前的研究表明，SRC是一种与太平洋白虾（Litopenaeus vannamei）相关的新型候选基因。为了理解LVSRC影响虾生长的潜在机制，我们分析了该基因在L. vannamei中的结构，系统发育，表达谱和RNA干扰（RNAi）。我们发现LVSRC包含两个CCP结构域和一个MAM结构域，在心脏中具有最高的表达水平，在其他组织中表达水平相对较低。值得注意的是，LVSRC在具有不同生长速率的群体之间的快速增长组中表现出明显更高的表达水平，这表明其潜在的参与是有助于Vannamei的生长的基因。与对照组相比，LVSRC的RNAi抑制了体长和体重增加。此外，通过RNA-seq分析，我们确定了598个差异表达的基因（DEG），包括与生长，免疫，蛋白质加工和修饰相关的基因，信号转导，脂质合成和代谢。此外，基因本体论（GO）和基因和基因组的京都百科全书（KEGG）分析显示，与生长，脂质代谢和免疫反应有关的信号传导途径发生了显着变化，这表明LVSRC表现出潜在参与多样性的物理学过程和免疫质量调节的潜力。。这些发现加深了我们对SRC在虾中的结构和功能的理解，并为进一步研究LVSRC的调节机制奠定了基础。此外，它们还提供了虾遗传学和育种的潜在应用。
Fuhua Li [a] [b] [c] [d]. Structure and function analyses of the SRC gene in Pacific white shrimp Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 2024,147: 109433