图书章节

Patch Pipettes (Micropipettes) 收藏

贴片移液器（微孔）

原文求助发布源

作者

Nicholas Graziane[1];Yan Dong[2];Nicholas Graziane[3];Yan Dong[4]

作者单位

[1]Penn State College of Medicine, Hershey, PA, USA;[2]University of Pittsburgh, Pittsburgh, PA, USA;[3]Department of Anesthesiology, The Pennsylvania State University, Hershey, PA, USA;[4]Neuroscience Department, University of Pittsburgh, Pittsburgh, PA, USA

关键词

Micropipette fabrication;Micropipette equipment;Micropipette resistance;Electrode holders;Pipette drift

关键词译文

微量移液器的制造；微型移动设备；微管电阻；电极支架；移液器漂移

页码

69-78

DOI

10.1007/978-1-0716-2589-7_5

来源信息

Electrophysiological Analysis of Synaptic Transmission EISBN：9781071625897, 2022年, 69-78页

摘要

Joining an established electrophysiology laboratory typically means that the micropipette fabrication process has already been perfected allowing newcomers to be trained without a complete understanding of how the current method was developed. This chapter looks to fill that knowledge gap by discussing available micropipette options and fabrication methods currently used for in vitro electrophysiology (e.g., inside-out, outside-out, whole-cell, cell-attached, and sharp-electrode patches). We also discuss the importance of the micropipette’s shape in relation to the in vitro electrophysiology model used (e.g., tissue slice, cell culture, dissociated neurons).

摘要译文

加入已建立的电生理实验室通常意味着已经完善了微型移动工艺，可以训练新移民，而无需完全了解当前方法的开发。本章旨在通过讨论当前用于体外电生理学的可用微目选项和制造方法来填补这一知识差距（例如，内外，外部，外部，全细胞，全细胞，细胞连接和尖锐的电极斑块）。我们还讨论了微目形状与所使用的体外电生理模型（例如，组织切片，细胞培养，解离神经元）的重要性。

Nicholas Graziane[1];Yan Dong[2];Nicholas Graziane[3];Yan Dong[4]. Patch Pipettes (Micropipettes). Electrophysiological Analysis of Synaptic Transmission[M].DE: Springer, 2022: 69-78