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Detection of Plant Viruses Through RNA-Seq 收藏

利用RNA-Seq技术检测植物病毒

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作者

作者单位

[1]Plant Virology and Molecular Pathology Laboratory, Division of Plant Pathology, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India;[2]Department of Plant Pathology, College of Horticulture, Dr. YSRHU, Bhimavaram, Andhra Pradesh, India;[3]Department of Fruit Science, College of Horticulture, Dr. YSRHU, Bhimavaram, Andhra Pradesh, India;[4]Division of Plant Pathology, Sher-e-Kashmir University of Agriculture Sciences and Technology of Kashmir (SKUAST-K), Srinagar, Jammu and Kashmir, India;[5]Division of Plant Pathology, Sher-e-Kashmir University of Agriculture Sciences and Technology of Kashmir (SKUAST-K), Shalimar, Jammu and Kashmir, India;[6]Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India;[7]Division of Genetics & Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India;[8]Division of Animal Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India

关键词

RNA-Seq;Viruses;Transcriptome;Selective depression of rRNA;RNA sequencing library

关键词译文

RNA-Seq；病毒；转录组；rRNA选择性抑制；RNA测序文库

发布日期

28 February 2025

页码

205-213

DOI

10.1007/978-1-0716-4390-7_31

来源信息

Detection of Plant Viruses Advanced Techniques ISBN：9781071643921, 2025年, 205-213页

摘要

RNA sequencing (RNA-seq) has revolutionized plant virus detection. This powerful technique offers an unbiased approach to identify a wide range of viruses, including those previously unknown. Unlike traditional methods targeting specific pathogens, RNA-seq analyzes the entire pool of RNA molecules within a plant sample. This comprehensive approach allows for the simultaneous detection of multiple viruses, even in mixed infections and also collects the host transcriptome data. In RNA-Seq experiment, removal of ribosomal RNA (rRNA) to enrich for viral RNA sequences is critical as these are overshadowed with mRNA. However, RNA-seq can detect viruses with varying genome types, including those with and without polyadenylated tails. Beyond detection, RNA-seq has the ability to not only identify viruses but also to explore host plant responses to infection through analysis of the host transcriptome data obtained along with viral sequences. This method briefly explains the careful depletion of ribosomal RNA (Sd rRNA) followed by RNA sequencing library preparation to detect a wide range of viruses.

摘要译文

RNA测序（RNA-seq）已经彻底改变了植物病毒的检测。这种强大的技术提供了一种无偏倚的方法来识别广泛的病毒，包括以前未知的病毒。与针对特定病原体的传统方法不同，RNA-seq分析植物样品中的整个RNA分子库。这种综合方法允许同时检测多种病毒，即使在混合感染中，也收集宿主转录组数据。在RNA-Seq实验中，去除核糖体RNA（rRNA）以富集病毒RNA序列至关重要，因为这些序列被mRNA遮蔽。然而，RNA-seq可以检测具有不同基因组类型的病毒，包括带有和不带有多聚腺苷酸化尾巴的病毒。除检测外，RNA-seq不仅能够鉴定病毒，而且还能够通过分析与病毒序列一起获得的宿主转录组数据来探索宿主植物对感染的应答。该方法简要解释了小心耗尽核糖体RNA（Sd rRNA），然后制备RNA测序文库以检测多种病毒。

Dasari Meghanath & Nulevino Iralu[1];Kottam Sushma[2];Rao Thulasi[3];Aflaq Hamid[4];Sumiah Wani[5];Aflaq Hamid [6];Gowhar Ali [7];Asif Shikari [7];Sahar Saleem [8];Shabir Hussain Wani [7];Sumiah Wani [6]. Detection of Plant Viruses Through RNA-Seq. Detection of Plant Viruses Advanced Techniques[M].DE: Springer, 2025: 205-213