摘要
Sequencing of RNA by next generation sequencers, RNA-Seq, is revolutionizing virus detection. In addition to the unbiased detection of various viruses from wild plants in natural environments, RNA-Seq also allows for the parallel collection of host plant transcriptome data. Host transcriptome data are highly valuable for studying the responses of hosts to viral infections, as well as viral host manipulation. When detecting viruses using RNA-Seq, it is critical to choose appropriate methods for the removal of rRNA from total RNA. Although viruses with polyadenylated genomes can be detected by RNA-Seq following mRNA purification using oligo-dT beads, viruses with non-polyadenylated genomes are not effectively detected. However, such viruses can be detected by RNA-Seq using the rRNA selective depression method. The high-throughput and cost-effective method of RNA-Seq library preparation which is described here allows us to detect a broad range of viruses in wild plants.
摘要译文
下一代测序仪RNA-SEQ的RNA测序正在旋转病毒检测。除了从自然环境中的野生植物中的各种病毒检测外，RNA-SEQ还允许并行收集宿主工厂转录组数据。宿主转录组数据对于研究宿主对病毒感染的反应以及病毒宿主操纵是非常有价值的。当使用RNA-SEQ检测病毒时，选择用于从总RNA中去除RRNA的合适方法至关重要。虽然可以通过使用寡聚珠珠的MRNA-SEQ通过RNA-SEQ检测具有聚腺苷酸化基因组的病毒，但是没有有效地检测具有非聚腺苷酸化基因组的病毒。然而，使用RNA-SEQ使用RRNA选择性抑制方法可以检测这些病毒。这里描述的RNA-SEQ库制剂的高通量和成本效益方法允许我们检测野生植物中的广泛病毒。
Atsushi J. Nagano...;Mie N. Honjo.;Motohiro Mihara.;Masanao Sato.;Hiroshi Kudoh...;Atsushi J. Nagano.1;2;Mie N. Honjo.1;Motohiro Mihara.3;Masanao Sato.4;Hiroshi Kudoh.1. Detection of Plant Viruses in Natural Environments by Using RNA-Seq. [M].Springer, 2015: 89-98