图书章节

Prime Editing for Precise Genome Engineering in Drosophila 收藏

果蝇精确基因组工程的主要编辑

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作者

Justin A. Bosch & Norbert Perrimon[1];Norbert Perrimon[2];Christian Dahmann[3]

作者单位

[1]Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA;[2]Howard Hughes Medical Institute, Chevy Chase, MD, USA;[3]Institute of Genetics, Technische Universität Dresden, Dresden, Germany

关键词

Prime editing;pegRNA;CRISPR;Genome engineering;Precise editing;\n Drosophila\n

关键词译文

Prime编辑； Pegrna; CRISPR;基因组工程；精确编辑； \ n果蝇\ n

页码

113-134

DOI

10.1007/978-1-0716-2541-5_5

来源信息

Drosophila ISBN：9781071625408, 2022年, 113-134页

摘要

Editing the Drosophila genome is incredibly useful for gene functional analysis. However, compared to gene knockouts, precise gene editing is difficult to achieve. Prime editing, a recently described CRISPR/Cas9-based technique, has the potential to make precise editing simpler and faster, and produce less errors than traditional methods. Initially described in mammalian cells, prime editing is functional in Drosophila somatic and germ cells. Here, we outline steps to design, generate, and express prime editing components in transgenic flies. Furthermore, we highlight a crossing scheme to produce edited fly stocks in less than 3 months.

摘要译文

编辑果蝇基因组对于基因功能分析非常有用。但是，与基因敲除相比，很难实现精确的基因编辑。Prime Editing是最近描述的基于CRISPR/CAS9的技术，具有使精确编辑更简单，更快的潜力，并且比传统方法更少。最初在哺乳动物细胞中描述的主要编辑在果蝇和生殖细胞中起作用。在这里，我们概述了在转基因苍蝇中设计，生成和表达主要编辑组件的步骤。此外，我们重点介绍了不到3个月内生产编辑的飞股的交叉计划。

Justin A. Bosch & Norbert Perrimon[1];Norbert Perrimon[2];Christian Dahmann[3]. Prime Editing for Precise Genome Engineering in Drosophila. Drosophila[M].DE: Springer, 2022: 113-134