摘要
The large-scale proteomic analysis of Dictyostelium discoideum has contributed to our understanding of intracellular as well as secreted proteins in this versatile model eukaryote. Mass spectrometry-based proteomic analysis is a robust, sensitive, and rapid analytical method for identification and characterization of proteins extracted from tissues, cells, cell fractions, or pull-down assays. The availability of core facilities which make proteomics inexpensive and easy to do has facilitated a wide range of research projects. In this chapter, we present a simple standard methodology to extract proteins and prepare samples from D. discoideum for mass spectrometry and methods to analyze the identified proteins.
摘要译文
Dictyostelium discoideum的大规模蛋白质组学分析有助于我们在这种多功能模型真核生物中对细胞内和分泌的蛋白质的理解。基于质谱的蛋白质组学分析是一种可靠，敏感和快速的分析方法，用于鉴定和表征从组织，细胞，细胞部分或下拉测定中提取的蛋白质。核心设施的可用性使蛋白质组学廉价且易于做，从而促进了广泛的研究项目。在本章中，我们提出了一种简单的标准方法，用于提取蛋白质并从D。Discoideum中制备样品，以进行质谱和分析鉴定蛋白质的方法。
Ramesh Rijal & Richard H. Gomer[1];Alan R. Kimmel [2]. Proteomic Analysis of Dictyostelium discoideum by Mass Spectrometry. Dictyostelium discoideum Methods and Protocols[M].DE: Springer, 2024: 247-255