摘要
Artificial insemination (AI) is the introduction of sperm into the female reproductive tract by means of an instrument. Several important developments, including novel sperm diluents, new AI techniques, protocols for synchronizing estrus and ovulation, and the availability of gender- selected semen, have increased the use and importance of AI as an assisted reproduction technique. Achieving high fertility with AI requires excellent management of all phases of the AI program. In that regard, personnel responsible for semen collection, processing and delivery must all correctly perform their tasks. Frozen semen is stored in a specialized tank containing liquid nitrogen. Various methods for thawing semen in straws have been recommended, including a variety of water bath temperatures and thawing periods, shirt pocket thawing, air-thawing, and thawing in the cow. Higher fertility requires proper insemination methods such as uterine body insemination, bilateral cornual insemination, deep uterine horn insemination, and intrafollicular insemination.
摘要译文
人工授精（AI）是引入精子进入女性生殖道通过仪器的装置。有几个重要的发展，包括新的精子稀释剂，新的人工智能技术，同步发情和排卵，并选择性别精液的可用性协议，增加了AI的使用和重要性作为辅助生殖技术。实现高生育率与AI需要AI程序的各个阶段优秀的管理。在这方面，负责精液采集，处理和送货人员都必须正确地执行他们的任务。冷冻精液储存在含有液氮的专用箱。各种方法在吸管解冻的精液已建议，包括多种水浴温度和解冻期间，衬衣口袋解冻，空气解冻，解冻的牛。肥力较高，需要适当的人工授精的方法，如子宫体受精，宫角双边人工授精，深子宫角输精和卵泡内人工授精。
Ram Kasimanickam. Artificial Insemination. Bovine Reproduction[M].US: wiley, 2014: 295-303