摘要
Corynebacterium glutamicum strain development has adopted each metabolic engineering tool immediately upon its conception and has contributed to developing these tools further. In the last more than five decades, C. glutamicum strains have been selected and screened after undirected mutagenesis. This approach yielded industrially relevant strains, however, it was limited by the little insight that could be gained hampering transfer to related processes. Genetic engineering, the complete genome sequence and omics tools, systems biology and synthetic biology have closed this gap and provided a profound knowledge base to develop engineering strategies driven by metabolic insight. Quite recently, serendipitous untargeted approaches came into focus again since mutants selected classically e.g. using genetically encoded biosensors or by adaptive laboratory evolution could be understood when genome resequencing was combined with genetic and biochemical experiments. We will discuss metabolic engineering from the classical, genetic engineering, systems biology eras to the era of synthetic biology for C. glutamicum strain development and forecast the impact of the most recent methods such as CRISPR technology and adaptive laboratory evolution.
摘要译文
谷氨酸棒杆菌菌株的开发在其构想之后就立即采用了每种代谢工程工具，并为进一步开发这些工具做出了贡献。在过去的五十多年里，谷氨酸棒杆菌菌株已经被选择并在无定向诱变后进行了筛选。这种方法产生了与工业相关的菌株，但是，由于很少的见识而受到阻碍，因为这些见识阻碍了向相关过程的转移。基因工程，完整的基因组序列和组学工具，系统生物学和合成生物学填补了这一空白，并提供了深厚的知识基础来开发由代谢洞察力驱动的工程策略。最近，由于突变体经典地选择了例如突变体，偶然的非靶向方法再次成为焦点。当基因组重测序与遗传和生化实验相结合时，可以理解使用遗传编码的生物传感器或通过适应性实验室进化。我们将讨论从经典，遗传工程，系统生物学时代到合成生物学时代的谷氨酸棒杆菌菌株开发的代谢工程，并预测诸如CRISPR技术和自适应实验室进化等最新方法的影响。
Volker F. Wendisch.1;2;Jin-Ho Lee.3. Metabolic Engineering in Corynebacterium glutamicum. Corynebacterium glutamicum[M].DE: Springer, 2020: 287-322