摘要
Leptin (Ob) was originally thought to be secreted exclusively by fat tissue regulating energy metabolism and satiety. Nowadays, it is known to be produced by many organs and to influence reproductive processes as well as other bodily functions. In the dog, little is known about Ob’s role in reproduction. To investigate the potential involvement of Ob in canine pregnancy, we determined the localization of Ob and its receptor (ObR) by in situ hybridization (ISH) and immunohistochemistry (IHC) in the corpus luteum (CL), uterus and placenta of pregnant bitches and followed the changes in their gene expression levels by RT- PCR. Tissues were obtained from bitches ovariohysterectomized during pre- and post- implantation, mid-gestation and prepartum luteolysis. Also, uteri were collected from early pregnant dogs and their matched non-pregnant controls in diestrus. Ob and ObR mRNA and protein were detected during all stages of pregnancy in various cell types of the CL, uterus and placenta. ObR mRNA expression was increased in the pre-implantation uterus and in inter-placental sections at post-implantation. At placentation sites Ob mRNA was highest during post-implantation and prepartum luteolysis, and ObR expression was up-regulated prepartum. In the non-pregnant diestrus uterus Ob mRNA was below the detection limit, and ObR was not different between the groups. In conclusion, Ob may be involved in the establishment and maintenance of canine pregnancy in a paracrine/autocrine manner
摘要译文
Leptin (Ob) was originally thought to be secreted exclusively by fat tissue regulating energy metabolism and satiety. Nowadays, it is known to be produced by many organs and to influence reproductive processes as well as other bodily functions. In the dog, little is known about Ob’s role in reproduction. To investigate the potential involvement of Ob in canine pregnancy, we determined the localization of Ob and its receptor (ObR) by in situ hybridization (ISH) and immunohistochemistry (IHC) in the corpus luteum (CL), uterus and placenta of pregnant bitches and followed the changes in their gene expression levels by RT- PCR. Tissues were obtained from bitches ovariohysterectomized during pre- and post- implantation, mid-gestation and prepartum luteolysis. Also, uteri were collected from early pregnant dogs and their matched non-pregnant controls in diestrus. Ob and ObR mRNA and protein were detected during all stages of pregnancy in various cell types of the CL, uterus and placenta. ObR mRNA expression was increased in the pre-implantation uterus and in inter-placental sections at post-implantation. At placentation sites Ob mRNA was highest during post-implantation and prepartum luteolysis, and ObR expression was up-regulated prepartum. In the non-pregnant diestrus uterus Ob mRNA was below the detection limit, and ObR was not different between the groups. In conclusion, Ob may be involved in the establishment and maintenance of canine pregnancy in a paracrine/autocrine manner
Staub, Livia Patricia. Localization and quantification of leptin and leptin receptor in the canine corpus luteum, placenta and uterus during pregnancy[D]. CH: University of Zurich, 2014