图书章节

Baculovirus Vectors 收藏

杆状病毒载体

原文求助发布源

作者

Azeem Ansari[1];Vincent C. Emery[1]

作者单位

[1]Department of VtrologyRoyal Free Hospttal School of MedicineUK

关键词

Herpes Simplex Virus ;Transfer Vector ;High Level Synthesis ;Transporter Associate With Antigen Presentation ;Mature Capsid

关键词译文

单纯疱疹病毒;转移载体;高水平合成;转运蛋白与抗原呈递相关;成熟衣壳

页码

219-233

DOI

10.1007/978-1-59259-642-3_19

来源信息

Molecular Biomethods Handbook ISBN：9780896035010, 1998年, 219-233页

摘要

Recombmant baculovinrus expression systems are now firmly established as one of the tools for producing foreign proteins in high yield wlthin a eukaryotlc environment The evolution of both single- and multiple-transfer vectors and different forms of baculovlral DNA has greatly streainlined the lsolatlon and purlficatlon of a recoinblnant virus carrying the foreign gene of interest. This chapter summarize the wealth of transfer vectors and baculovnus DNAs available to the researcher to generate both single- and multiple-expressing recombinant baculovnuses and highlights the specific areas in which we feel that recombinant protein expressed via the baculovnus system has enabled siginficant advances to be inade

摘要译文

重组杆状病毒表达系统现已被确立为在真核环境中以高产率生产外源蛋白质的工具之一。单转移载体和多转移载体以及不同形式的杆状DNA的进化极大地增强了llolatlon和purlficatlon的作用。携带外源基因的重新携带病毒。本章总结了研究人员可用于产生单表达和多表达重组杆状病毒的转移载体和杆状病毒DNA的丰富性，并突出了我们认为通过杆状病毒系统表达的重组蛋白使得siginficant进展不足的特定区域。

Azeem Ansari[1];Vincent C. Emery[1]. Baculovirus Vectors. Molecular Biomethods Handbook[M].DE: Springer, 1998: 219-233