图书章节

Hybridization of Environmental Microbial Community Nucleic Acids by GeoChip 收藏

GeoChip环境微生物群落核酸杂交

原文求助

作者

Joy D. Van Nostrand1Huaqin Yin1Liyou Wu1Tong Yuan1Jizhong Zhou1Email author

作者单位

1.Institute for Environmental Genomics and Department of Microbiology and Plant BiologyUniversity of OklahomaNormanUSA

关键词

GeoChip Functional gene array Microbial communities Microbial ecology Hybridization Fluorescent labeling Whole community genome amplification

关键词译文

GeoChip功能基因阵列微生物群落微生物生态学杂交荧光标记全社区基因组扩增

发布日期

21 January 2016

页码

183-196

来源信息

Microbial Environmental Genomics (MEG) ISBN：9781493933679, 2016年, 183-196页

摘要

Functional gene arrays, like the GeoChip, allow for the study of tens of thousands of genes in a single assay. The GeoChip array (5.0) contains probes for genes involved in geochemical cycling (N, C, S, and P), metal homeostasis, stress response, organic contaminant degradation, antibiotic resistance, secondary metabolism, and virulence factors as well as genes specific for fungi, protists, and viruses. Here, we briefly describe GeoChip design strategies (gene selection and probe design) and discuss minimum quantity and quality requirements for nucleic acids. We then provide detailed protocols for amplification, labeling, and hybridization of samples to the GeoChip.

摘要译文

功能基因阵列，如GeoChip，允许在单个测定中研究数万个基因。GeoChip阵列（5个包含涉及地球化学循环（N，C，S和P）的基因的探针，金属体内平衡，应激反应，有机污染物降解，抗生素耐药性，次生代谢和毒力因子以及真菌，原生生物和病毒特异性基因。我们简要介绍了GeoChip设计策略（基因选择和探针设计），并讨论了最小量和然后提供核酸的质量要求。然后为GeoChip then提供样品的扩增，标记和杂交的详细方案

Joy D. Van Nostrand1Huaqin Yin1Liyou Wu1Tong Yuan1Jizhong Zhou1Email author. Hybridization of Environmental Microbial Community Nucleic Acids by GeoChip. Microbial Environmental Genomics (MEG)[M].DE: Springer, 2016: 183-196