摘要
Joining an established electrophysiology laboratory typically means that the micropipette fabrication process has already been perfected allowing newcomers to be trained without a complete understanding of how the current method was developed. This chapter looks to fill that knowledge gap by discussing available micropipette options and fabrication methods currently used for in vitro electrophysiology (e.g., inside-out, outside-out, whole-cell, cell-attached, and sharp-electrode patches). We also discuss the importance of the micropipette’s shape in relation to the in vitro electrophysiology model used (e.g., tissue slice, cell culture, dissociated neurons). For laboratories setting-up electrophysiology equipment, we have provided information regarding tools needed for pulling micropipettes as well as post-pulling fabrication processes (e.g., fire polishing). Finally, we discuss micropipette drift, which can occur during electrophysiological recordings accompanied by a micropipette-drift troubleshooting guide. Surprisingly, the process of micropipette fabrication can play a significant role in data collection. With this in mind, we anticipate by the end of this chapter that the reader will have the information necessary to fabricate the appropriate micropipette for their desired electrophysiology preparation allowing for successful data collection.
摘要译文
修饰典型地意味着微量移液器制造过程已经完善，允许新手在没有完全理解当前方法如何被开发的情况下被训练本章通过讨论目前用于体外电生理学的可用微量吸移管选项和制造方法（例如从内向外，外向外，全细胞，细胞附着和尖电极贴片）。我们还讨论了微量移液器形状与使用的体外电生理学模型的关系的重要性（e竒 组织切片，细胞培养，解离神经元） 对于实验室设置电生理学设备，我们提供了有关拉动微量移液器以及后拉制造工艺（电火花抛光）所需工具的信息。最后，我们讨论微量移液器漂移，这可以在伴随着微量移液器故障排除指南的电生理记录期间发生。令人惊奇的是，微量移液器制造的过程可以在数据收集中发挥重要作用。考虑到这一点，本章的读者将有必要的信息制造适当的微量移液器为他们所需的电生理学准备允许成功的数据收集
Nicholas Graziane (4); Yan Dong (4);. Patch Pipettes (Micropipettes). Electrophysiological Analysis of Synaptic Transmission[M].DE: Springer, 2016: 69-78