摘要
A baculovirus expression vector (BEV) is a recombinant baculovirus with a double-stranded circular DNA genome that has been genetically modified to include a foreign gene of interest. BEVs are viable and can infect susceptible hosts, usually cultured lepidopteran insect cells or larvae, in a helper-independent fashion. Therefore, BEVs can efficiently transfer foreign genes into these eukaryotic host cells. The foreign gene is usually a chimeric construct with the sequence encoding a protein of interest placed under the transcriptional control of a viral promoter. This arrangement enables viral functions to transcribe the gene during infection. The resulting mRNA is translated and the newly synthesized protein modified by host-encoded biosynthetic machinery. In essence, then, BEVs and their insect cell hosts are two separate components of a binary eukaryotic expression system, which will be called simply the BEV system throughout the remainder of this chapter.
摘要译文
杆状病毒表达载体（BEV）是具有双链环状DNA基因组的重组杆状病毒，其已经被遗传修饰以包含外源基因。 BEV是可行的并且可以以独立于辅助的方式感染易感宿主，通常是培养的鳞翅目昆虫细胞或幼虫。因此，BEV可以有效地将外源基因转移到这些真核宿主细胞中。外源基因通常是嵌合构建体，其编码感兴趣的蛋白质的序列置于病毒启动子的转录控制下。这种安排使得病毒功能能够在感染期间转录基因。得到的mRNA被翻译，并且由宿主编码的生物合成机器修饰新合成的蛋白质。从本质上说，BEVs和它们的昆虫细胞宿主是二元真核表达系统的两个独立的组成部分，在本章剩下的部分将简单地称为BEV系统。
Donald L. Jarvis1. Baculovirus Expression Vectors. The Baculoviruses[M].DE: Springer, 1997: 389-431