摘要
The alkaline phosphatase (AP) characteristics of three algal bloom species in the coastal waters of China [Prorocentrum donghaiense D. Lu, Alexandrium catenella (Whedon et Kof.) Balech, and Skeletonema costatum (Grev.) Cleve] were analyzed in a laboratory batch culture experiment using bulk assay and the single-cell enzyme-labeled fluorescence (ELF) method. Results showed that the AP of these three test species shared some common characteristics: AP was inducible in all three species and was expressed by algae under phosphorus (P)–stress conditions; no constitutive AP enzyme was detected in the three test species. Once AP was produced, all three test species gradually released the enzymes into the water, and the algae would reinduce AP production. There were also different specific AP characteristics among the three test species under severe P-stressed conditions. In P. donghaiense, AP covered most of the cell, and the AP production sites were mainly on the cell surface, although some could be observed inside cells. AP also covered the whole cell of A. catenella, but the AP sites were mainly inside the cell with only some on the cell surface. Only one or two AP sites could be detected in S. costatum, and they were all on the cell surface.
摘要译文
碱性磷酸酶（AP）三种赤潮种在中国沿海水域的特点[东海原甲藻D.路，链状亚历山大藻（温登等拳皇）Balech，和中肋骨条藻（GREV。）克利夫]是在一个实验室分批培养实验使用批量法和单细胞酶标记荧光（ELF）方法进行分析。结果表明，这三种试验物种的该AP共享一些共同的特点：AP是诱导在所有三个物种和下磷（P）-stress条件由藻类表达;在这三个试验物种中没有检测到的AP组成的酶。一旦AP被生产，所有三个测试品种逐步释放酶入水，和海藻将reinduce AP的生产。也有严重的P-强调条件下的三个测试物种之间不同的具体特点AP。在东海原甲藻，AP覆盖大部分的细胞，和AP生产地点，主要是在细胞表面上，尽管一些可能在细胞内被观察到。 AP还涵盖A. catenella的全细胞，但AP位点主要是只有某些细胞表面上的细胞内。只有一个或两个AP位点可以在中肋骨条藻被检测到，并且它们均在细胞表面上。
Linjian Ou[1]; Bangqin Huang[2,‡ Huasheng Hong[2]; Yuzao Qi[3] and Songhui Lu[3];. COMPARATIVE ALKALINE PHOSPHATASE CHARACTERISTICS OF THE ALGAL BLOOM DINOFLAGELLATES PROROCENTRUM DONGHAIENSE AND ALEXANDRIUM CATENELLA, AND THE DIATOM SKELETONEMA COSTATUM1[J]. Journal of Phycology, 2010,46(2): 260-265