摘要
Probiotics represent a possible strategy for controlling intestinal infections in livestock. Members of the Weissella genus are increasingly being studied for health-related applications in animals and humans. Here we investigated the functional properties of two Weissella cibaria strains isolated from cows reared in Kuwait breeding facilities by combining phenotypic with genomic analyses. W. cibaria SP7 and SP19 exhibited good growth in vitro under acidic conditions and in the presence of bile salts compared to the reference probiotic Lacticaseibacillus (formerly Lactobacillus) rhamnosus GG. Both strains were able to adhere to Caco-2 and HT-29 cell lines, as well as to mucin. The cell-free supernatants of the two isolates exhibited inhibitory activity towards Escherichia coli ATCC 25,922 and Salmonella enterica UC3605, which was ultimately due to the low pH of supernatants. W. cibaria SP19 showed a co-aggregation ability similar to that of L. rhamnosus GG when incubated with S. enterica. Whole genome sequencing and analysis revealed that both strains harbored several genes involved in carbohydrate metabolism and general stress responses, indicating bacterial adaptation to the gastrointestinal environment. We also detected genes involved in the adhesion to host epithelial cells or extracellular matrix. No evidence of acquired antibiotic resistance or hemolytic activity was found in either strain. These findings shed light on the potential of W. cibaria for probiotic use in livestock and on the mechanisms underlying host-microbe interaction in the gut. W. cibaria` strain SP19 exhibited the best combination of in vitro probiotic properties and genetic markers, and is a promising candidate for further investigation.
摘要译文
益生菌代表了控制牲畜中肠道感染的可能策略。越来越多地研究卫生酱属的成员，用于动物和人类的健康相关应用。在这里，我们研究了从科威特育种设施中饲养的母牛分离的两种Weissella Cibaria菌株的功能性质，通过与基因组分析相结合。 W.Cibaria SP7和SP19在酸性条件下呈现出良好的体外生长，并且与参考益生菌乳酸乳腈（以前乳酸杆菌）rhamOosus Gg相比，胆汁盐存在良好。两种菌株能够粘附到Caco-2和HT-29细胞系中，以及粘蛋白。两种分离物的无细胞上清液表现出对大肠杆菌ATCC 25,922和沙门氏体肠溶UC3605的抑制活性，这最终是由于上清液的低pH值。 W. Cibaria SP19显示了与L. rhamoosus Gg的共聚能力，当与S.肠道孵育时。全基因组测序和分析表明，两种菌株都有几种参与碳水化合物代谢和一般应激反应的基因，表明细菌适应胃肠环境。我们还检测到涉及宿主上皮细胞或细胞外基质的粘附性的基因。在任一个菌株中发现没有获得抗生素抗性或溶血活性的证据。这些调查结果阐明了在牲畜中益生菌使用的W.Cibaria的潜力和肠道中宿主微生物相互作用的机制。 W. Cibaria`菌株SP19表现出体外益生菌性质和遗传标记的最佳组合，是进一步调查的有希望的候选者。
Vania Patrone[1];Tahani Al-Surrayai[2];Francesco Romaniello[3];Alessandra Fontana[1];Giovanni Milani[1];Valeria Sagheddu[4];Edoardo Puglisi[1];Maria Luisa Callegari[3];Hamad Al-Mansour[2];Mohamed Waheed Kishk[2];Lorenzo Morelli[1]. Integrated Phenotypic-Genotypic Analysis of Candidate Probiotic Weissella Cibaria Strains Isolated from Dairy Cows in Kuwait[J]. Probiotics and Antimicrobial Proteins, 2021,13(3): 809-823